Morphological Evaluation of Wound Healing Events in the Excisional Wound Healing Model in Rats
Ligia EStevão,
Puebla Cassini-Vieira,
Ana Greice Leite,
Apolônia Bulhões,
Lucíola Barcelos,
Joaquim Evêncio-Neto
Affiliations
Ligia EStevão
Department of Morphology and Animal Physiology, Universidade Federal Rural de Pernambuco (UFRPE), Recife-PE, Brazil
Puebla Cassini-Vieira
Department of Medicine, Division of Hematology/Oncology Department of Immunology and Microbiology, Cancer Center Rush University Medical Center, Chicago, USA
Ana Greice Leite
Department of Morphology and Animal Physiology, Universidade Federal Rural de Pernambuco (UFRPE), Recife-PE, Brazil
Apolônia Bulhões
Department of Morphology and Animal Physiology, Universidade Federal Rural de Pernambuco (UFRPE), Recife-PE, Brazil
Lucíola Barcelos
Department of Physiology and Biophysics (ICB) Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil
Joaquim Evêncio-Neto
Department of Morphology and Animal Physiology, Universidade Federal Rural de Pernambuco (UFRPE), Recife-PE, Brazil
Skin wound healing is a complex process involving different events such as blood coagulation, inflammation, new blood vessels formation, and extracellular matrix deposition. These events can be observed by using histology techniques. However, the lack of the standardization of such parameters impacts on the reproducibility of results. Here, we describe a protocol to perform macroscopic and microscopic analyses of the events that occur during skin wound healing using the experimental model of excisional wounds in rats.
