Bio-Protocol (Jul 2019)

Morphological Evaluation of Wound Healing Events in the Excisional Wound Healing Model in Rats

  • Ligia EStevão,
  • Puebla Cassini-Vieira,
  • Ana Greice Leite,
  • Apolônia Bulhões,
  • Lucíola Barcelos,
  • Joaquim Evêncio-Neto

DOI
https://doi.org/10.21769/BioProtoc.3285
Journal volume & issue
Vol. 9, no. 13

Abstract

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Skin wound healing is a complex process involving different events such as blood coagulation, inflammation, new blood vessels formation, and extracellular matrix deposition. These events can be observed by using histology techniques. However, the lack of the standardization of such parameters impacts on the reproducibility of results. Here, we describe a protocol to perform macroscopic and microscopic analyses of the events that occur during skin wound healing using the experimental model of excisional wounds in rats.