Tanyptera (Tanyptera) hebeiensis Yang et Yang (Diptera: Tipulidae) newly recorded from Shandong, China: sequencing and phylogenetic analysis of the mitochondrial genome

Abstract

The genus Tanyptera Latreille, is recorded from Shandong Province, China for the first time with T. (T.) hebeiensis Yang et Yang, found in Mount Kunyu, Shandong. In this study, we report the complete mitochondrial genome sequence of T. (T.) hebeiensis, representing the first mitochondrial genome of the subfamily Ctenophorinae (Diptera: Tipulidae), which is a circular molecule of 15,888 bp with an AT content of 77.6%. The mitochondrial genome contains 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and a non-coding region. Gene overlaps are found at nine gene junctions, ranging from 1 to 8 bp in length. The canonical mitochondrial start codons for invertebrate mitochondrial genomes are found in 12 PCGs, except for COI which uses the uncanonical start codons TCG. Stop codons of 10 PCGs are invariably complete TAA and TAG, while COII, ND4, and ND5 end with a single thymine stop codon. Phylogenetic analysis reveals that the Pediciidae is a sister group to the remaining Tipuloidea, the Cylindrotomidae has a sister-group relationship with the Tipulidae, and the Limoniidae is not a monophyletic clade.

Keywords

• new record
• mitogenome
• phylogeny
• ctenophorinae
• crane-fly