Generation of transgenic mice expressing a FRET biosensor, SMART, that responds to necroptosis

Shin Murai; Kanako Takakura; Kenta Sumiyama; Kenta Moriwaki; Kenta Terai; Sachiko Komazawa-Sakon; Takao Seki; Yoshifumi Yamaguchi; Tetuo Mikami; Kimi Araki; Masaki Ohmuraya; Michiyuki Matsuda; Hiroyasu Nakano

doi:10.1038/s42003-022-04300-0

Communications Biology (Dec 2022)

Generation of transgenic mice expressing a FRET biosensor, SMART, that responds to necroptosis

Shin Murai,
Kanako Takakura,
Kenta Sumiyama,
Kenta Moriwaki,
Kenta Terai,
Sachiko Komazawa-Sakon,
Takao Seki,
Yoshifumi Yamaguchi,
Tetuo Mikami,
Kimi Araki,
Masaki Ohmuraya,
Michiyuki Matsuda,
Hiroyasu Nakano

Affiliations

Shin Murai: Department of Biochemistry, Toho University School of Medicine, 5-21-16 Omori-Nishi, Ota-ku
Kanako Takakura: Imaging Platform for Spatio-Temporal Regulation, Graduate School of Medicine, Kyoto University
Kenta Sumiyama: Laboratory for Mouse Genetic Engineering, RIKEN Center for Biosystems Dynamics Research
Kenta Moriwaki: Department of Biochemistry, Toho University School of Medicine, 5-21-16 Omori-Nishi, Ota-ku
Kenta Terai: Research Center for Dynamic Living Systems, Graduate School of Biostudies, Kyoto University
Sachiko Komazawa-Sakon: Department of Biochemistry, Toho University School of Medicine, 5-21-16 Omori-Nishi, Ota-ku
Takao Seki: Department of Biochemistry, Toho University School of Medicine, 5-21-16 Omori-Nishi, Ota-ku
Yoshifumi Yamaguchi: Hibernation metabolism, physiology, and development Group, Environmental Biology Division, Institute of Lo600-601. Temperature Science, Hokkaido University, Kita 19, Nishi 8, Kita-ku
Tetuo Mikami: Department of Pathology, Toho University School of Medicine, 5-21-16 Omori-Nishi, Ota-ku
Kimi Araki: Center for Animal Resources and Development, Kumamoto University, 2–2–1 Honjo, Chuo-ku
Masaki Ohmuraya: Department of Genetics, Hyogo College of Medicine
Michiyuki Matsuda: Research Center for Dynamic Living Systems, Graduate School of Biostudies, Kyoto University
Hiroyasu Nakano: Department of Biochemistry, Toho University School of Medicine, 5-21-16 Omori-Nishi, Ota-ku

DOI: https://doi.org/10.1038/s42003-022-04300-0
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 14

Abstract

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The SMART (MLKL-activation reporter) system FRET biosensor allows for necroptosis assessment in primary cells and in a mouse model of cisplatininduced AKI.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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