Kandungan Total Polifenol dan Aktivitas Antioksidan dari Ekstrak Metanol Akar Imperata cylindrica (L) Beauv. (Alang-alang)

Abstract

Hypertension is caused by many factors, including by the activity of angiotensin converting enzyme (ACE), reactive oxygen species (ROS). Methanol extract of Imperata cylindrica (alang-alang) root has been proven as having anti-hypertensive activities. Study shows various antioxidant therapies can decrease blood pressure. Polyphenol compounds of plants have many benefits, including as an antioxidant. Therefore, an experimental study was performed to measure the total polyphenol content using visible spectrophotometry method-Folin-Ciocalteu reagent as well as to test the antioxidant activity using 1,1-diphenyl-method 2-pikrilhidrazil (DPPH) from July to December 2014. The results showed that the extract had a total polyphenol content of 1.53% galad acid aquevalent (GAE) and antioxidant activity IC50 0.32 mg/mL. The polyphenol compounds have the ability to donate hydrogen atom to DPPH free radical, which leads to reduced DPPHmarked by the color change of DPPH from purple to yellow. Thus, antioxidant activity of methanol extract of Imperata cylindrica root was supported by the presence of polyphenol compounds. In conclusion, the extract has a total polyphenol content of 1.53% (GAE) and antioxidant activity IC50 0.32 of 0.32 mg/mL. The presence of polyphenol compounds supports the antioxidant activity of the extract.

Keywords

• Antioxidant activity
• Imperata cylindrica
• total polyphenol content