Single-cell temporal analysis of natural dengue infection reveals skin-homing lymphocyte expansion one day before defervescence
Jantarika Kumar Arora,
Anunya Opasawatchai,
Tiraput Poonpanichakul,
Natnicha Jiravejchakul,
Waradon Sungnak,
Oranart Matangkasombut,
Sarah A. Teichmann,
Ponpan Matangkasombut,
Varodom Charoensawan,
Anavaj Sakuntabhai,
Pratap Singhasivanon,
Swangjit Suraamornkul,
Tawatchai Yingtaweesak,
Khajohnpong Manopwisedjaroen,
Nada Pitabut
Affiliations
Jantarika Kumar Arora
Doctor of Philosophy Program in Biochemistry (International Program), Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Anunya Opasawatchai
Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand; Integrative Computational Bioscience (ICBS) Center, Mahidol University, Nakorn Pathom 73170, Thailand
Tiraput Poonpanichakul
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Systems Biology of Diseases Research Unit, Faculty of Science Mahidol University, Bangkok 10400, Thailand
Natnicha Jiravejchakul
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Waradon Sungnak
Integrative Computational Bioscience (ICBS) Center, Mahidol University, Nakorn Pathom 73170, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Wellcome Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Oranart Matangkasombut
Department of Microbiology and Center of Excellence on Oral Microbiology and Immunology, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand; Research Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok 10210, Thailand
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Systems Biology of Diseases Research Unit, Faculty of Science Mahidol University, Bangkok 10400, Thailand; Corresponding author
Varodom Charoensawan
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Integrative Computational Bioscience (ICBS) Center, Mahidol University, Nakorn Pathom 73170, Thailand; Systems Biology of Diseases Research Unit, Faculty of Science Mahidol University, Bangkok 10400, Thailand; Corresponding author
Summary: Effective clinical management of acute dengue virus (DENV) infection relies on the timing of suitable treatments during the disease progression. We analyzed single-cell transcriptomic profiles of the peripheral blood mononuclear cell samples from two DENV patients, collected daily during acute phase and also at convalescence. Key immune cell types demonstrated different dynamic responses over the course of the infection. On the day before defervescence (Day −1), we observed the peak expression of several prominent genes in the adaptive immunological pathways. We also characterized unique effector T cell clusters that expressed skin-homing signature genes at Day −1, whereas upregulation of skin and gut homing genes was also observed in plasma cells and plasmablasts during the febrile period. This work provides an overview of unique molecular dynamics that signify the entry of the critical phase, and the findings could improve the patient management of DENV infection.
