Overexpression of CrSMT gene enhances salt stress tolerance by improving cotton peroxidation resistance
Peilin Wang,
Xiurong Tan,
Weilong Li,
Xinyue Xu,
Chenhui Li,
Wenfang Guo,
Xiaofeng Su,
Hongmei Cheng,
Huiming Guo
Affiliations
Peilin Wang
National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, 572024, China; Biotechnology Research Institute/Key Laboratory of Agricultural Microbiome (MARA), Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Xiurong Tan
Biotechnology Research Institute/Key Laboratory of Agricultural Microbiome (MARA), Chinese Academy of Agricultural Sciences, Beijing, 100081, China; College of Life Sciences, Zhejiang Normal University, Jinhua, 321004, Zhejiang, China
Weilong Li
National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, 572024, China; Biotechnology Research Institute/Key Laboratory of Agricultural Microbiome (MARA), Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Xinyue Xu
National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, 572024, China; Biotechnology Research Institute/Key Laboratory of Agricultural Microbiome (MARA), Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Chenhui Li
National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, 572024, China; Biotechnology Research Institute/Key Laboratory of Agricultural Microbiome (MARA), Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Wenfang Guo
Biotechnology Research Institute/Key Laboratory of Agricultural Microbiome (MARA), Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Xiaofeng Su
Biotechnology Research Institute/Key Laboratory of Agricultural Microbiome (MARA), Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Hongmei Cheng
National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, 572024, China; Biotechnology Research Institute/Key Laboratory of Agricultural Microbiome (MARA), Chinese Academy of Agricultural Sciences, Beijing, 100081, China; Corresponding authors.
Huiming Guo
National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, 572024, China; Biotechnology Research Institute/Key Laboratory of Agricultural Microbiome (MARA), Chinese Academy of Agricultural Sciences, Beijing, 100081, China; Corresponding authors.
Salt stress is an important abiotic stress factor affecting crop production and plant geographical distribution. Salt stress negatively impacts molecular, biochemical, and physiological processes in cotton, resulting in inhibition of plant growth and development and, in severe cases, plant death. In this experiment, the CrSMT gene isolated from the unicellular eukaryote Chlamydomonas reinhardtii was overexpressed in cotton R15. Two transgenic lines, L17 and L25, were obtained. Treated with 200 mM NaCl experiments showed that the CrSMT-transgenic cotton had enhanced tolerance to salt stress. RNA-seq analysis revealed that CrSMT overexpression in cotton resulted in the synthesis of a large number of secondary metabolites responsive to salt stress. Correlation analysis between the wild type and the transgenic lines revealed that CrSMT overexpression did not affect the growth, agronomic traits, and fiber quality of cotton. The function of CrSMT holds potential to improve plant tolerance of abiotic stress factors.
