Generation and characterization of a novel human iPSC line from a resilient Alzheimer’s disease patient

Debamitra Das; Jiaxin Li; Senquan Liu; Esther Oh; Linzhao Cheng; Constantine Lyketsos; Vasiliki Mahairaki

Stem Cell Research (Oct 2020)

Generation and characterization of a novel human iPSC line from a resilient Alzheimer’s disease patient

Debamitra Das,
Jiaxin Li,
Senquan Liu,
Esther Oh,
Linzhao Cheng,
Constantine Lyketsos,
Vasiliki Mahairaki

Affiliations

Debamitra Das: Department of Neurology, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA
Jiaxin Li: Department of Neurology, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA
Senquan Liu: Division of Hematology, Department of Medicine, and the Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore MD 21287, USA
Esther Oh: Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA; The Richman Family Precision Medicine Center of Excellence in Alzheimer's Disease, Johns Hopkins Medicine and Johns Hopkins Bayview Medical Center, Baltimore, MD, USA
Linzhao Cheng: Division of Hematology, Department of Medicine, and the Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore MD 21287, USA
Constantine Lyketsos: The Richman Family Precision Medicine Center of Excellence in Alzheimer's Disease, Johns Hopkins Medicine and Johns Hopkins Bayview Medical Center, Baltimore, MD, USA; The Department of Psychiatry and Behavioral Sciences, Johns Hopkins Medicine and Johns Hopkins Bayview Medical Center, Baltimore, MD, USA
Vasiliki Mahairaki: Department of Neurology, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA; The Richman Family Precision Medicine Center of Excellence in Alzheimer's Disease, Johns Hopkins Medicine and Johns Hopkins Bayview Medical Center, Baltimore, MD, USA; Corresponding author at: Department of Neurology, Johns Hopkins University School of Medicine, USA.

Journal volume & issue: Vol. 48
p. 101979

Abstract

Read online

Alzheimer’s disease (AD) is a progressive neurodegenerative disease that is the major cause of dementia in older people. Here, we report the derivation of human induced pluripotent stem cells (iPSCs) from an AD patient at age of 80 who has the APOE ε4/ε4 genotype and is resilient to cognitive decline for 10 years. The iPSCs reprogrammed from the blood cells of this patient by transient expression of pluripotency genes maintain the ε4/ε4 genotype, are karyotypically normal and display typical iPSC characteristics. Upon differentiation, the iPSCs are able to differentiate into cells of the three germ layers, confirming their pluripotency.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal