Titanium Disc Modified with Hydroxyapatite and Zirconia Does not Reveal Signs of Cytotoxicity

Abstract

The paper deals primarily with the study of biological properties of a model of implant covered with a thin layer of hydroxyapatite with an intermediate layer of zirconium. Cytotoxicity was evaluated by direct cultivation with mouse and human fibroblasts. A physical test using X-ray diffraction confirmed that the film is composed of crystalline hydroxyapatite. The evaluation of the cytotoxicity test and a comparison with controlled samples cultured in parallel in vitro made it possible to conclude that the material is not cytotoxic audit is suitable for further experiments in more detail.

Keywords

• implant
• hydroxyapatite
• zirconium
• x-ray diffraction
• direct cytotoxicity test