Isolation and identification of the alkaloids from rhizomes of Stephania macrantha
Guofeng SUN,
Fengzheng CHEN,
Chong TIAN,
Ying CHENG,
Shuhua LI
Affiliations
Guofeng SUN
School of New Energy Materials and Chemistry, Leshan Engineering Research Center for Medicinal Components of Characteristic Agro-Products, Leshan Normal University, Leshan 614000, Sichuan, China
Fengzheng CHEN
School of New Energy Materials and Chemistry, Leshan Engineering Research Center for Medicinal Components of Characteristic Agro-Products, Leshan Normal University, Leshan 614000, Sichuan, China
Chong TIAN
School of New Energy Materials and Chemistry, Leshan Engineering Research Center for Medicinal Components of Characteristic Agro-Products, Leshan Normal University, Leshan 614000, Sichuan, China
Ying CHENG
School of New Energy Materials and Chemistry, Leshan Engineering Research Center for Medicinal Components of Characteristic Agro-Products, Leshan Normal University, Leshan 614000, Sichuan, China
Shuhua LI
School of New Energy Materials and Chemistry, Leshan Engineering Research Center for Medicinal Components of Characteristic Agro-Products, Leshan Normal University, Leshan 614000, Sichuan, China
To study the alkaloids in the rhizomes of Stephania macrantha. The total alkaloids of S. macrantha were extracted by acid extraction and alkali precipitation method. Eleven alkaloids have been isolated and purified by silica gel column chromatography and preparative high performance liquid chromatography (HPLC). The structures of compounds were identified by spectroscopic methods (NMR and MS). The results were as follows: (1) Eleven compounds were designated as sinomenine (1), sinoactine (2), stepharine (3), reticuline (4), isocorydine (5), corydalmine (6), asimilobine (7), sukhodianine (8), dicentrine (9), 7-oxocrebanine (10) and palmatine (11). (2) The total alkaloids of S. macrantha and sinomenine had inhibitory activities on human lung cancer cells (A549), with IC50 values of 7.5×10-4 g·mL-1 and 6.59×10-9 g·mL-1, respectively. Compounds 2, 3, 4, 7, 8, 9 and 10 were isolated for the first time from S. macrantha. The chemical constituents from S. macrantha belong to five types of alkaloids such as morphanes, proaporphines, aporphines, benzyltetrahydroi soquinolines and protoberberines.
