Phoxim-specific DNA aptamer screening, characterization and application in a multiple complementary strands fluorescent aptasensor

Wenfei Guo; Dan Wei; Franklin Wang-Ngai Chow; Polly Hang-Mei Leung; Hanming Wang; Lei Cai; Masahi Hori; Zhu Chen; Song Li; Yan Deng

Smart Materials in Medicine (Jan 2022)

Phoxim-specific DNA aptamer screening, characterization and application in a multiple complementary strands fluorescent aptasensor

Wenfei Guo,
Dan Wei,
Franklin Wang-Ngai Chow,
Polly Hang-Mei Leung,
Hanming Wang,
Lei Cai,
Masahi Hori,
Zhu Chen,
Song Li,
Yan Deng

Affiliations

Wenfei Guo: Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou, 412007, PR China
Dan Wei: Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou, 412007, PR China
Franklin Wang-Ngai Chow: Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong, 999077, PR China
Polly Hang-Mei Leung: Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong, 999077, PR China
Hanming Wang: Guangzhou Wondfo Biotech Co., Ltd., Guangzhou, 510663, PR China
Lei Cai: Guangzhou Wondfo iCubate Biotech Co., Ltd, Guangzhou, 510641, PR China
Masahi Hori: FNW Inc, Tokyo, 135-0051, Japan
Zhu Chen: Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou, 412007, PR China
Song Li: Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou, 412007, PR China; Corresponding author.
Yan Deng: Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou, 412007, PR China; Corresponding author.

Journal volume & issue: Vol. 3
pp. 289 – 296

Abstract

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In order to develop a fluorometric aptamer-based biosensor for ultrasensitive and selective detection of phoxim, a modified magnetic beads-systematic evolution of ligands by exponential enrichment (MB-SELEX) method was used in this study to select phoxim aptamer. An aptamer (APT3) that bonded to phoxim with high affinity was obtained after 15 rounds of selection, which had dissociation constant (Kd) of 0.93 ± 0.25 μM. A fluorometric aptasensor was then developed and enhanced the sensitivity and selectivity of phoxim detection based on APT3, multi-complementary strands (CS) and gold nanoparticles (AuNPs). This method had a good linear range of 0.05–5 μM, and detection limit was as low as 29.69 nM for phoxim. In addition, the aptasensing specifically captured phoxim in a variety of pesticides and real samples. The herein results proved that the method can be applied in practical detection.

Published in Smart Materials in Medicine

ISSN: 2590-1834 (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology
Website: https://www.keaipublishing.com/en/journals/smart-materials-in-medicine/

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