Nasal cytology as a reliable non-invasive procedure to phenotype patients with type 2 chronic rhinosinusitis with nasal polyps

Giovanni Paoletti, MD; Luca Malvezzi, MD; Anna Maria Riccio, BSc; Desideria Descalzi, BSc; Francesca Pirola, MD; Elena Russo, MD; Laura De Ferrari, BSc; Francesca Racca, MD; Sebastian Ferri, MD; Maria Rita Messina, MD; Francesca Puggioni, MD; Emanuele Nappi, MD; Diego Bagnasco, MD; Frank Rikki Canevari, MD; Fabio Grizzi, BSc; Giuseppe Mercante, MD; Giuseppe Spriano, MD; Giorgio Walter Canonica, MD; Enrico Heffler, MD

World Allergy Organization Journal (Nov 2022)

Nasal cytology as a reliable non-invasive procedure to phenotype patients with type 2 chronic rhinosinusitis with nasal polyps

Giovanni Paoletti, MD,
Luca Malvezzi, MD,
Anna Maria Riccio, BSc,
Desideria Descalzi, BSc,
Francesca Pirola, MD,
Elena Russo, MD,
Laura De Ferrari, BSc,
Francesca Racca, MD,
Sebastian Ferri, MD,
Maria Rita Messina, MD,
Francesca Puggioni, MD,
Emanuele Nappi, MD,
Diego Bagnasco, MD,
Frank Rikki Canevari, MD,
Fabio Grizzi, BSc,
Giuseppe Mercante, MD,
Giuseppe Spriano, MD,
Giorgio Walter Canonica, MD,
Enrico Heffler, MD

Affiliations

Giovanni Paoletti, MD: Personalized Medicine, Asthma and Allergy, IRCCS Humanitas Research Hospital, via Manzoni 56, 20089 Rozzano, Milan, Italy; Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, MI, Italy; Corresponding author. Personalized Medicine, Asthma and Allergy, Humanitas Clinical and Research Center IRCCS, Rozzano, MI, Italy. Istituto Clinico Humanitas, Rozzano, MI, Italy.
Luca Malvezzi, MD: Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, MI, Italy; Department of Otorhinolaryngology and Head and Neck Surgery, IRCCS Humanitas Research Hospital, Rozzano, MI, Italy
Anna Maria Riccio, BSc: Allergy and Respiratory Diseases, IRCCS Policlinico San Martino, Genova, Italy; Department of Internal Medicine (DIMI), University of Genova, Genova, Italy
Desideria Descalzi, BSc: Personalized Medicine, Asthma and Allergy, IRCCS Humanitas Research Hospital, via Manzoni 56, 20089 Rozzano, Milan, Italy; Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, MI, Italy
Francesca Pirola, MD: Department of Otorhinolaryngology and Head and Neck Surgery, IRCCS Humanitas Research Hospital, Rozzano, MI, Italy
Elena Russo, MD: Department of Otorhinolaryngology and Head and Neck Surgery, IRCCS Humanitas Research Hospital, Rozzano, MI, Italy
Laura De Ferrari, BSc: Allergy and Respiratory Diseases, IRCCS Policlinico San Martino, Genova, Italy; Department of Internal Medicine (DIMI), University of Genova, Genova, Italy
Francesca Racca, MD: Personalized Medicine, Asthma and Allergy, IRCCS Humanitas Research Hospital, via Manzoni 56, 20089 Rozzano, Milan, Italy
Sebastian Ferri, MD: Personalized Medicine, Asthma and Allergy, IRCCS Humanitas Research Hospital, via Manzoni 56, 20089 Rozzano, Milan, Italy
Maria Rita Messina, MD: Personalized Medicine, Asthma and Allergy, IRCCS Humanitas Research Hospital, via Manzoni 56, 20089 Rozzano, Milan, Italy; Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, MI, Italy
Francesca Puggioni, MD: Personalized Medicine, Asthma and Allergy, IRCCS Humanitas Research Hospital, via Manzoni 56, 20089 Rozzano, Milan, Italy
Emanuele Nappi, MD: Personalized Medicine, Asthma and Allergy, IRCCS Humanitas Research Hospital, via Manzoni 56, 20089 Rozzano, Milan, Italy; Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, MI, Italy
Diego Bagnasco, MD: Allergy and Respiratory Diseases, IRCCS Policlinico San Martino, Genova, Italy; Department of Internal Medicine (DIMI), University of Genova, Genova, Italy
Frank Rikki Canevari, MD: Othorinolaryngology, IRCCS Policlinico San Martino, Genova, Italy; Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genova, Genova, Italy
Fabio Grizzi, BSc: Department of Immunology and Inflammation, IRCCS Humanitas Research Hospital, Rozzano, 20089, Milan, Italy
Giuseppe Mercante, MD: Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, MI, Italy; Department of Otorhinolaryngology and Head and Neck Surgery, IRCCS Humanitas Research Hospital, Rozzano, MI, Italy
Giuseppe Spriano, MD: Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, MI, Italy; Department of Otorhinolaryngology and Head and Neck Surgery, IRCCS Humanitas Research Hospital, Rozzano, MI, Italy
Giorgio Walter Canonica, MD: Personalized Medicine, Asthma and Allergy, IRCCS Humanitas Research Hospital, via Manzoni 56, 20089 Rozzano, Milan, Italy; Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, MI, Italy
Enrico Heffler, MD: Personalized Medicine, Asthma and Allergy, IRCCS Humanitas Research Hospital, via Manzoni 56, 20089 Rozzano, Milan, Italy; Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, MI, Italy

Journal volume & issue: Vol. 15, no. 11
p. 100700

Abstract

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Background: The identification of type-2 inflammation in patients with chronic rhinosinusitis with nasal polyps (CRSwNP) acquires a crucial role in the endotypization needed for selecting patients for biological drugs targeting type-2 inflammation: to date, the parameters used include systemic and histological biomarkers. The aim of this study was to investigate whether nasal cytology could identify type-2 inflammation in patients with CRSwNP. Methodology: Thirty-three consecutive patients with CRSwNP underwent nasal cytology sampling at the level of the lower nasal turbinate, and of the polypoid tissue, and surgical polyp tissue sample was collected. The cellularity of the 3 collected samples were compared. Results: Mean nasal polyp tissue, nasal polyps cytology and inferior turbinate cytology eosinophils counts were 43.7 ± 39.6 cells/HPF, 32.8 ± 44.7 cells/HPF and 27.6 ± 58.0 cells/HPF respectively with inferior turbinate cytology eosinophils significantly lower than nasal polyp tissue count (p = 0.007). Both mean nasal polyps cytology eosinophils and mean inferior turbinate cytology eosinophils were significantly higher in patients with type-2 CRSwNP (52.5 ± 67.0 cells/HPF vs 12.2 ± 17.3 cells/HPF, p = 0.012, and 32.0 ± 62.1 cells/HPF vs 2.9 ± 2.9 cells/HPF, p = 0.020 respectively). Conclusions: Nasal cytology is suitable tool for assessing local biomarkers of type-2 inflammation in CRSwNP.

Published in World Allergy Organization Journal

ISSN: 1939-4551 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Immunologic diseases. Allergy
Website: https://www.journals.elsevier.com/world-allergy-organization-journal/

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