Molecular detection of antimicrobial resistance genes among ciprofloxacin-resistant Pseudomonas aeruginosa isolates

Abstract

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen that is considered one of the most important causes of nosocomial infections, especially in burns and immunocompromised individuals. So this study was aimed todetection of quinolone-resistant genes among ciprofloxacin-resistant Pseudomonas aeruginosa isolates. Data showed out of 168 specimens obtained from burns patients the rate women and men in this study were 107(63..69%) and 61(36.3%) respectively, positive bacterial growth were 159 (94.64 %) while 9(5.3%) of specimens were no growth. According to result of the vitek-2 system recorded 75 isolates as P. aeruginosa. Results of ciprofloxacin susceptibility recorded 29(38.67%) of P.auroginosa was resistance to ciprofloxacin, while was 34(45.33%), and 12(16%) of isolates were intermediate and sensitive respectively. Results of antibiotic susceptibility showed that the highest bacterial resistance was imipenem 29(100%), while the least resistance were meropenem and Piperacillin-Tazobactam reached 22(75.8%). Results of Polymerase chain reaction (PCR) showed that 29(100%), 28 (96.06%), 26(89.65%), 23(79.31%) and 21(72.41%) of ciprofloxacin-resistant Pseudomonas aeruginosa isolates were harbored aac(6’)-Ib, parC, qnrS, qnrB and qnrVC respectively, while qnrA, qnrC, qnrD, and qepA genes were not detect in present study. Sequence results for qnrB, qnrvc showed that they are identical to qnrB2, qnrvc1 when compared with international NCBI isolates.

Keywords

• molecular
• antimicrobial resistance
• ciprofloxacin-resistant
• pseudomonas aeruginosa