Інтродукція Рослин (Jun 2019)
Antioxidant activity of seed extracts of selected forage plants
Abstract
Objective – to investigate the antioxidant activity of seed extracts of selected forage plants in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Material and methods. Seeds of seven forage plants researched in this study: Baptisia australis (L.) R. Br. (Fabaceae), Bunias erucago L., B. orientalis L. (Brassicaceae), Galega officinalis L., G. orientalis Lam. (Fabaceae), Isatis littoralis Steven, I. tinctoria L. (Brassicaceae). The method described by Brand-Williams et al. (1995) used to determine the antiradical activity of plant extracts. Biochemical analyze included following stages: preparation of methanol, ethanol and water extracts (1 g of dried material mixed with 25 ml of solvent); 12 hours of extraction; spectrophotometric procedure of determination of antiradical activity with a 2,2-diphenyl-2-picrylhydrazyl solution. A solution of radical prepared in methanol and diluted according to method. Data were expressed in ascorbic acid equivalent (AAE) and Trolox equivalent (TE) on a gram of dry weight. Measurements of extracts carried out on spectrophotometer UNICO UV 2800 at 515 nm. Experimental data processed by Excel. Results. Methanol extracts of investigated plants had an antiradical activity of 18.27–80.57 %, ethanol extracts of 11.07–79.73 % and water extracts of 23.31–80.26 %. Antioxidant activity of methanol extracts expressed on ascorbic acid equivalent was 1.75–2.72 mg AAE per gram, ethanol extracts 1.62–2.72 mg AAE per gram and water extracts 1.82–2.66 mg AAE per gram. Results exhibited antioxidant activity expressed on Trolox equivalent of methanol extracts from 1.30 to 7.51 mg TE per gram, ethanol extracts from 0.56 to 7.76 mg TE per gram and water extracts from 1.19 to 7.37 mg TE per gram. Conclusions. Obtained data demonstrated that in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine seed extracts of investigated forage plants have high antioxidant potential as well as leaves or shoots. Antiradical activity of extracts was of 11.07–84.60 %. All seed extracts had the least values of this parameter in the ethanolic extracts. Antioxidant activity expressed in ascorbic acid equivalent was maximal for Bunias orientalis and Galega officinalis and minimal for Isatis tinctoria. Antioxidant activity by Trolox equivalent was high for Bunias orientalis methanol extracts and slow for Bunias erucago ethanol extracts. Investigation of seeds extracts of crops from different plant families is need to evaluate antioxidant potential of it and recommend for use.
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