Frontiers in Environmental Science (Nov 2022)
Pretreatment of NaCl enhances the drought resistance of cotton by regulating the biosynthesis of carotenoids and abscisic acid
- Kesong Ni,
- Maohua Dai,
- Xuke Lu,
- Yuexin Zhang,
- Yapeng Fan,
- Nan Xu,
- Xixian Feng,
- Hui Huang,
- Jing Wang,
- Cun Rui,
- Hong Zhang,
- Yuqian Lei,
- Xiaoyu Liu,
- Tiantian Jiang,
- Mingge Han,
- Liangqing Sun,
- Xiugui Chen,
- Delong Wang,
- Junjuan Wang,
- Shuai Wang,
- Chao Chen,
- Lixue Guo,
- Lanjie Zhao,
- Wuwei Ye
Affiliations
- Kesong Ni
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Maohua Dai
- Hebei Academy of Agricultural and Forestry Sciences/Hebei Key Laboratory of Crops Drought Resistance, Dryland Farming Institute, Hengshui, Hebei, China
- Xuke Lu
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Yuexin Zhang
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Yapeng Fan
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Nan Xu
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Xixian Feng
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Hui Huang
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Jing Wang
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Cun Rui
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Hong Zhang
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Yuqian Lei
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Xiaoyu Liu
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Tiantian Jiang
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Mingge Han
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Liangqing Sun
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Xiugui Chen
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Delong Wang
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Junjuan Wang
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Shuai Wang
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Chao Chen
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Lixue Guo
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Lanjie Zhao
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- Wuwei Ye
- State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, China
- DOI
- https://doi.org/10.3389/fenvs.2022.998141
- Journal volume & issue
-
Vol. 10
Abstract
Drought stress is one of the abiotic stresses that limits crop production and greatly affects crop yield. Enhancement of plant stress resistance by NaCl pretreatment has been reported, but the mechanism by which NaCl pretreatment activates cotton stress resistance remains unclear. In this study, upland cotton (Gossypium hirsutum cv H177) was used as the material to conducted the treatments with three replications: 0 Mm NaCl + 0% PEG6000 (Polyethylene glycol), 0 mM NaCl + 15% PEG6000, 50 mM NaCl + 15% PEG6000 to explore the molecular mechanism by which NaCl improves the drought tolerance of cotton. The results showed that pretreatment with 50 mM NaCl could alleviate the adverse effects of PEG on cotton seeds while promoting the elongation of root length. RNA-seq showed that NaCl specifically induced the expression of carotenoid-related genes. By silencing the upstream gene GHLUT2 of lutein synthesis, it was found that the chlorophyll of silenced plants decreased, and leaf wilting was more sensitive to drought. We found that NaCl enhanced the drought resistance of cotton by regulating genes related to the carotenoid and abscisic acid downstream synthesis pathways. This study provides a new reference for the study of drought resistance in cotton and a theoretical basis for the molecular breeding of cotton.
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