Development of an optical sensor based on surface plasmon resonance phenomenon for diagnosis of dengue virus E-protein

Nur Alia Sheh Omar; Yap Wing Fen; Jaafar Abdullah; Che Engku Noramalina Che Engku Chik; Mohd Adzir Mahdi

Sensing and Bio-Sensing Research (Sep 2018)

Development of an optical sensor based on surface plasmon resonance phenomenon for diagnosis of dengue virus E-protein

Nur Alia Sheh Omar,
Yap Wing Fen,
Jaafar Abdullah,
Che Engku Noramalina Che Engku Chik,
Mohd Adzir Mahdi

Affiliations

Nur Alia Sheh Omar: Functional Devices Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
Yap Wing Fen: Functional Devices Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Department of Physics, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Corresponding author at: Functional Devices Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Jaafar Abdullah: Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
Che Engku Noramalina Che Engku Chik: Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
Mohd Adzir Mahdi: Wireless and Photonics Network Research Centre, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

Journal volume & issue: Vol. 20
pp. 16 – 21

Abstract

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Application of surface plasmon resonance (SPR) optical sensor in the diagnosis of dengue virus (DENV) emerged over recent years. Immobilized monoclonal antibody (IgM) on gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film was prepared using a spin coating technique. DENV E-protein can be detected by measuring the SPR signal when IgM immobilized gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film is exposed to the DENV E-protein solution, by varying the concentration ranging from 0.0001 nM to 10 nM. A linear relationship between the shift of SPR angle and concentration of DENV E-protein up to 0.01 nM, with sensitivity of 39.96° nM−1 has been observed. The surface morphology of sensor chip was also recorded using atomic force microscopy to confirm the presence of bound DENV E-protein into IgM surface. Keywords: Optical sensor, Surface plasmon resonance, Sensing, Dengue virus

Published in Sensing and Bio-Sensing Research

ISSN: 2214-1804 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Engineering (General). Civil engineering (General)
Website: http://www.journals.elsevier.com/sensing-and-bio-sensing-research/

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