Cell culture NAIL-MS allows insight into human tRNA and rRNA modification dynamics in vivo

Matthias Heiss; Felix Hagelskamp; Virginie Marchand; Yuri Motorin; Stefanie Kellner

doi:10.1038/s41467-020-20576-4

Nature Communications (Jan 2021)

Cell culture NAIL-MS allows insight into human tRNA and rRNA modification dynamics in vivo

Matthias Heiss,
Felix Hagelskamp,
Virginie Marchand,
Yuri Motorin,
Stefanie Kellner

Affiliations

Matthias Heiss: Department of Chemistry, Ludwig-Maximilians-University Munich
Felix Hagelskamp: Department of Chemistry, Ludwig-Maximilians-University Munich
Virginie Marchand: Université de Lorraine, CNRS, Inserm, UMS2008/US40 IBSLor and UMR7365 IMoPA
Yuri Motorin: Université de Lorraine, CNRS, Inserm, UMS2008/US40 IBSLor and UMR7365 IMoPA
Stefanie Kellner: Department of Chemistry, Ludwig-Maximilians-University Munich

DOI: https://doi.org/10.1038/s41467-020-20576-4
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

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Post transcriptional modification of RNAs represents an important layer of gene regulation. Here the authors describe NAIL-MS—a method for monoisotopic RNA labeling in cell culture—demonstrating its capabilities by analyzing the modification kinetics of total tRNA, 18S rRNA and tRNAPhe as models.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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