Nature Communications (Aug 2018)

Structural insights into the gating of DNA passage by the topoisomerase II DNA-gate

  • Shin-Fu Chen,
  • Nan-Lan Huang,
  • Jung-Hsin Lin,
  • Chyuan-Chuan Wu,
  • Ying-Ren Wang,
  • Yu-Jen Yu,
  • Michael K. Gilson,
  • Nei-Li Chan

DOI
https://doi.org/10.1038/s41467-018-05406-y
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 13

Abstract

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Type II DNA topoisomerases (Top2s) direct the passage of one DNA duplex through another, which is important for resolving DNA entanglements. Here the authors combine X-ray crystallography and MD simulations and present the structure of the human Top2 DNA-gate in an open conformation and discuss mechanistic implications.