Detection of breast cancer cells by a near-infrared fluorescent probe targeting mitochondrial viscosity
Yu Shi,
Junjun Liu,
Yingying Liu,
Hong Quan,
Bo Li,
Haili Lu,
Hanzhi Ding,
Zuoren Yu,
Jing Han
Affiliations
Yu Shi
Department of Breast Cancer, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China
Junjun Liu
Department of Breast Cancer, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China
Yingying Liu
Department of Physiology and Pathophysiology, Health Science Center, Peking University, Beijing 100191, China
Hong Quan
Department of Breast Cancer, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China
Bo Li
Department of Breast Cancer, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China
Haili Lu
Department of Breast Cancer, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China
Hanzhi Ding
Department of Breast Cancer, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China
Zuoren Yu
Research Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China; Corresponding author.
Jing Han
Department of Breast Cancer, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China; Corresponding author.
Monitoring abnormal viscosity in biological systems is important for basic research and clinical applications. Fluorescence imaging technology is adaptable for the visualization of tumor tissues due to its comprehensive features. However, fluorescence detection of intracellular viscosity in clinical samples remains challenging. We developed a promising near-infrared fluorescent probe, M556, for viscosity measurement. M556, which targets mitochondria, was successfully applied to monitor the mitochondrial viscosity in living cells. Furthermore, M556 was demonstrated to effectively discriminate tumors from normal tissues in a mouse tumor model and in clinical specimens from breast cancer patients, thus indicating the potential perioperative use of this probe by clinicians to assist with biopsy procedures.
