Time-lapse single-cell transcriptomics reveals modulation of histone H3 for dormancy breaking in fission yeast

Hayato Tsuyuzaki; Masahito Hosokawa; Koji Arikawa; Takuya Yoda; Naoyuki Okada; Haruko Takeyama; Masamitsu Sato

doi:10.1038/s41467-020-15060-y

Nature Communications (Mar 2020)

Time-lapse single-cell transcriptomics reveals modulation of histone H3 for dormancy breaking in fission yeast

Hayato Tsuyuzaki,
Masahito Hosokawa,
Koji Arikawa,
Takuya Yoda,
Naoyuki Okada,
Haruko Takeyama,
Masamitsu Sato

Affiliations

Hayato Tsuyuzaki: Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University
Masahito Hosokawa: Research Organization for Nano and Life Innovation, Waseda University
Koji Arikawa: Computational Bio Big-Data Open Innovation Laboratory (CBBD-OIL), National Institute of Advanced Industrial Science and Technology
Takuya Yoda: Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University
Naoyuki Okada: Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University
Haruko Takeyama: Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University
Masamitsu Sato: Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University

DOI: https://doi.org/10.1038/s41467-020-15060-y
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 11

Abstract

Read online

Asynchronicity in fungal spore germination makes transcriptomic analysis of the process challenging. Here, the authors assay single cell transcriptomes of germinating yeast cells and find that one of the histone H3 genes shows fluctuating expression, disruption of which causes germination defects.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal