The Microalga <i>Skeletonema marinoi</i> Induces Apoptosis and DNA Damage in K562 Cell Line by Modulating NADPH Oxidase
Roberto Ciarcia,
Consiglia Longobardi,
Gianmarco Ferrara,
Serena Montagnaro,
Emanuela Andretta,
Francesco Pagnini,
Salvatore Florio,
Lucianna Maruccio,
Chiara Lauritano,
Sara Damiano
Affiliations
Roberto Ciarcia
Department of Veterinary Medicine and Animal Productions, University of Naples “Federico II”, Via Delpino n.1, 80137 Naples, Italy
Consiglia Longobardi
Department of Mental, Physical Health and Preventive Medicine, University of Campania “Luigi Vanvitelli”, Largo Madonna delle Grazie n.1, 80138 Naples, Italy
Gianmarco Ferrara
Department of Veterinary Medicine and Animal Productions, University of Naples “Federico II”, Via Delpino n.1, 80137 Naples, Italy
Serena Montagnaro
Department of Veterinary Medicine and Animal Productions, University of Naples “Federico II”, Via Delpino n.1, 80137 Naples, Italy
Emanuela Andretta
Department of Veterinary Medicine and Animal Productions, University of Naples “Federico II”, Via Delpino n.1, 80137 Naples, Italy
Francesco Pagnini
Department of Medicine and Surgery, Unit of Radiology, University of Parma, Via Università n. 12, 43126 Parma, Italy
Salvatore Florio
Department of Veterinary Medicine and Animal Productions, University of Naples “Federico II”, Via Delpino n.1, 80137 Naples, Italy
Lucianna Maruccio
Department of Veterinary Medicine and Animal Productions, University of Naples “Federico II”, Via Delpino n.1, 80137 Naples, Italy
Chiara Lauritano
Ecosustainable Marine Biotechnology Department, Stazione Zoologica Anton Dohrn, Via Acton n. 55, 80133 Naples, Italy
Sara Damiano
Department of Veterinary Medicine and Animal Productions, University of Naples “Federico II”, Via Delpino n.1, 80137 Naples, Italy
Chronic myeloid leukemia (CML) is a myeloproliferative disease that activates multiple signaling pathways, causing cells to produce higher levels of reactive oxygen species (ROS). Nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOXs) are a major generator of ROS in leukemia, and marine natural products have shown promising activities for the treatment of hematopoietic malignancies. In the present study, we investigated the effect of the marine microalga Skeletonema marinoi (S.M.), a ubiquitous diatom that forms massive blooms in the oceans, on the human leukemia cell line K562. The effects of S.M. extract on cell viability, production of ROS, nitric oxide (NO), and apoptosis were examined. In this preliminary work, S.M. was able to decrease cell viability (p p p p p < 0.05) in the K562-treated cells.
