Cancer Management and Research (Oct 2020)
Propofol Inhibits Proliferation and Invasion of Stomach Cancer Cells by Regulating miR-205/YAP1 Axis
Abstract
Xiang-Shu Xian,1 Yu-Tie Wang,2 Xiao-Meng Jiang3 1Department of Gastroenterology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Qingdao 264000, People’s Republic of China; 2Department of Rheumatology and Immunology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Qingdao 264000, People’s Republic of China; 3Department of Digestive, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, 211166, People’s Republic of ChinaCorrespondence: Xiao-Meng JiangDepartment of Digestive, Sir Run Run Hospital, Nanjing Medical University, Nanjing Jiangsu Province 211166, People’s Republic of ChinaEmail [email protected]: Propofol is a common clinical intravenous anesthetic. In the last few years, studies have revealed that propofol not only has good anesthetic effect but also has certain anticancer effect. However, its role in stomach cancer (SC) and related mechanisms are still under investigation.Objective: This study was designed to determine the effect of propofol on SC and its related mechanisms.Methods: Purchased SC cells were treated with propofol at different concentrations (5, 10, and 20 μg/mL), miR-205 overexpression, and YAP1 inhibition. Then, the Cell Counting Kit-8 (CCK8), Transwell, and flow cytometry were carried out to determine the biological behavior changes of treated cells and the expression of miR-205 and YAP1 after treatment.Results: Propofol (10 μg/mL and 20 μg/mL) inhibited the growth of SC cells and promoted their apoptosis, and overexpressing miR-205 or inhibiting YAP1 can exert the same effects. In addition, propofol (10μg/mL and 20μg/mL) up-regulated miR-205 in SC cells. The dual-luciferase reporter assay revealed that YAP1 could be targeted and regulated by miR-205, and the rescue assay revealed that inhibiting miR-205 or overexpressing YAP1 could weaken the effect of propofol on the biological behaviors of SC cells.Conclusion: Propofol can strongly suppress the proliferation and invasion of SC cells and induce their apoptosis via the miR-205/YAP1 axis.Keywords: propofol, stomach cancer, miR-205, YAP1, cell biology
