The effect of a fungal preparation on the tissue cultures of Holarrhena anfidysenterica (Roxb.) Wall.

Abstract

The addition of an aqueous extract from fruitbodies of Tylopilus felleus to tissue cultures of Holarrhena antidysenterica (Apocynaceae) caused the accumulation of an unknown compound in the culture medium. The compound was isolated and identified as 5-hydroxymethyl-2-furancarboxaldehyde (1). Moreover, biosynthesis of phenolic compounds was stimulated in response to the stress agents of the fungal preparation. Methyl ferulate (2) was the major phenolic constituent.

Keywords

• Holarrhena antidysenterica (Roxb.) Wall.
• tissue culture
• fungal preparation
• stress metabolites