Protein corona formation on epigallocatechin gallate-Au nanoparticles suppressed tumor accumulation
Chihiro Wakayama,
Sachiko Inubushi,
Tomonari Kunihisa,
Sachiko Mizumoto,
Motoi Baba,
Hirokazu Tanino,
Ik Sung Cho,
Tooru Ooya
Affiliations
Chihiro Wakayama
Graduate School of Engineering, Department of Chemical Science and Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657 8501, Japan
Sachiko Inubushi
Division of Breast and Endocrine Surgery, Graduate School of Medicine, Kobe University, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan
Tomonari Kunihisa
Division of Breast and Endocrine Surgery, Graduate School of Medicine, Kobe University, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan
Sachiko Mizumoto
Division of Breast and Endocrine Surgery, Graduate School of Medicine, Kobe University, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan
Motoi Baba
Division of Breast and Endocrine Surgery, Graduate School of Medicine, Kobe University, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan
Hirokazu Tanino
Division of Breast Surgery, Department of Surgery, Wakayama Medical University, 811-1 Kimiidera, Wakayama-shi, 641-8509, Japan
Ik Sung Cho
Department of Bioengineering, University of Illinois at Chicago, 1200 W Harrison St, Chicago, IL, 60607, USA
Tooru Ooya
Graduate School of Engineering, Department of Chemical Science and Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657 8501, Japan; Center for Advanced Medical Engineering Research & Development (CAMED), Kobe University, 1-5-1 Minatojima-minamimachi, Chuoku, Kobe, 657-0047, Japan; Corresponding author. Graduate School of Engineering, Department of Chemical Science and Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657 8501, Japan.
Metal nanoparticles (NPs), such as gold NPs (AuNPs), are particularly sensitive to X-rays, and thus specific accumulation of AuNPs in a tumor would allow radiotherapy with low energy X-rays and reduced side effects. AuNPs can be generated using HAuCl4 and the natural polyphenol epigallocatechin-3-gallate (EGCG) in the presence of citrate. Here, we generated EGCG-AuNPs in the presence of several additives and examined the accumulation of these NPs in mouse tumors following intravenous administration. EGCG-AuNPs 15 nm in diameter in the presence of sodium alginate accumulated more in tumors compared to 40-nm-diameter EGCG-AuNPs. Furthermore, the results of in vitro cellular uptake and serum protein absorption studies suggest that adsorption of 15–16 kDa serum proteins to EGCG-AuNPs suppresses accumulation in tumors. Thus, tendency to adsorb specific proteins on EGCG-AuNPs surface should be tailored for enhancing their accumulation in tumors.
