Protein corona formation on epigallocatechin gallate-Au nanoparticles suppressed tumor accumulation

Chihiro Wakayama; Sachiko Inubushi; Tomonari Kunihisa; Sachiko Mizumoto; Motoi Baba; Hirokazu Tanino; Ik Sung Cho; Tooru Ooya

JCIS Open (Apr 2023)

Protein corona formation on epigallocatechin gallate-Au nanoparticles suppressed tumor accumulation

Chihiro Wakayama,
Sachiko Inubushi,
Tomonari Kunihisa,
Sachiko Mizumoto,
Motoi Baba,
Hirokazu Tanino,
Ik Sung Cho,
Tooru Ooya

Affiliations

Chihiro Wakayama: Graduate School of Engineering, Department of Chemical Science and Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657 8501, Japan
Sachiko Inubushi: Division of Breast and Endocrine Surgery, Graduate School of Medicine, Kobe University, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan
Tomonari Kunihisa: Division of Breast and Endocrine Surgery, Graduate School of Medicine, Kobe University, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan
Sachiko Mizumoto: Division of Breast and Endocrine Surgery, Graduate School of Medicine, Kobe University, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan
Motoi Baba: Division of Breast and Endocrine Surgery, Graduate School of Medicine, Kobe University, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan
Hirokazu Tanino: Division of Breast Surgery, Department of Surgery, Wakayama Medical University, 811-1 Kimiidera, Wakayama-shi, 641-8509, Japan
Ik Sung Cho: Department of Bioengineering, University of Illinois at Chicago, 1200 W Harrison St, Chicago, IL, 60607, USA
Tooru Ooya: Graduate School of Engineering, Department of Chemical Science and Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657 8501, Japan; Center for Advanced Medical Engineering Research & Development (CAMED), Kobe University, 1-5-1 Minatojima-minamimachi, Chuoku, Kobe, 657-0047, Japan; Corresponding author. Graduate School of Engineering, Department of Chemical Science and Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657 8501, Japan.

Journal volume & issue: Vol. 9
p. 100074

Abstract

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Metal nanoparticles (NPs), such as gold NPs (AuNPs), are particularly sensitive to X-rays, and thus specific accumulation of AuNPs in a tumor would allow radiotherapy with low energy X-rays and reduced side effects. AuNPs can be generated using HAuCl4 and the natural polyphenol epigallocatechin-3-gallate (EGCG) in the presence of citrate. Here, we generated EGCG-AuNPs in the presence of several additives and examined the accumulation of these NPs in mouse tumors following intravenous administration. EGCG-AuNPs 15 nm in diameter in the presence of sodium alginate accumulated more in tumors compared to 40-nm-diameter EGCG-AuNPs. Furthermore, the results of in vitro cellular uptake and serum protein absorption studies suggest that adsorption of 15–16 kDa serum proteins to EGCG-AuNPs suppresses accumulation in tumors. Thus, tendency to adsorb specific proteins on EGCG-AuNPs surface should be tailored for enhancing their accumulation in tumors.

Published in JCIS Open

ISSN: 2666-934X (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Chemistry: Physical and theoretical chemistry; Technology: Chemical technology
Website: https://www.journals.elsevier.com/jcis-open

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