In this paper, we study 2-complex symmetric composition operators with the conjugation J, defined by Jf(z)=(f(z¯))¯, on the Hardy space H2. More precisely, we obtain the necessary and sufficient condition for the composition operator Cϕ to be 2-complex symmetric with J when ϕ is an automorphism of D. We also characterize 2-complex symmetric with J when ϕ is a linear fractional self-map of D.