Nature Communications (May 2022)
Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes
- Jeremy Vicencio,
- Carlos Sánchez-Bolaños,
- Ismael Moreno-Sánchez,
- David Brena,
- Charles E. Vejnar,
- Dmytro Kukhtar,
- Miguel Ruiz-López,
- Mariona Cots-Ponjoan,
- Alejandro Rubio,
- Natalia Rodrigo Melero,
- Jesús Crespo-Cuadrado,
- Carlo Carolis,
- Antonio J. Pérez-Pulido,
- Antonio J. Giráldez,
- Benjamin P. Kleinstiver,
- Julián Cerón,
- Miguel A. Moreno-Mateos
Affiliations
- Jeremy Vicencio
- Modeling human diseases in C. elegans Group; Genes, Disease and Therapy Program, Institut d’Investigació Biomèdica de Bellvitge - IDIBELL
- Carlos Sánchez-Bolaños
- Andalusian Center for Developmental Biology (CABD), Pablo de Olavide University/CSIC/Junta de Andalucía
- Ismael Moreno-Sánchez
- Andalusian Center for Developmental Biology (CABD), Pablo de Olavide University/CSIC/Junta de Andalucía
- David Brena
- Modeling human diseases in C. elegans Group; Genes, Disease and Therapy Program, Institut d’Investigació Biomèdica de Bellvitge - IDIBELL
- Charles E. Vejnar
- Department of Genetics, Yale University School of Medicine
- Dmytro Kukhtar
- Modeling human diseases in C. elegans Group; Genes, Disease and Therapy Program, Institut d’Investigació Biomèdica de Bellvitge - IDIBELL
- Miguel Ruiz-López
- Modeling human diseases in C. elegans Group; Genes, Disease and Therapy Program, Institut d’Investigació Biomèdica de Bellvitge - IDIBELL
- Mariona Cots-Ponjoan
- Modeling human diseases in C. elegans Group; Genes, Disease and Therapy Program, Institut d’Investigació Biomèdica de Bellvitge - IDIBELL
- Alejandro Rubio
- Andalusian Center for Developmental Biology (CABD), Pablo de Olavide University/CSIC/Junta de Andalucía
- Natalia Rodrigo Melero
- Biomolecular Screening and Protein Technologies Unit, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
- Jesús Crespo-Cuadrado
- Andalusian Center for Developmental Biology (CABD), Pablo de Olavide University/CSIC/Junta de Andalucía
- Carlo Carolis
- Biomolecular Screening and Protein Technologies Unit, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
- Antonio J. Pérez-Pulido
- Andalusian Center for Developmental Biology (CABD), Pablo de Olavide University/CSIC/Junta de Andalucía
- Antonio J. Giráldez
- Department of Genetics, Yale University School of Medicine
- Benjamin P. Kleinstiver
- Center for Genomic Medicine, Massachusetts General Hospital
- Julián Cerón
- Modeling human diseases in C. elegans Group; Genes, Disease and Therapy Program, Institut d’Investigació Biomèdica de Bellvitge - IDIBELL
- Miguel A. Moreno-Mateos
- Andalusian Center for Developmental Biology (CABD), Pablo de Olavide University/CSIC/Junta de Andalucía
- DOI
- https://doi.org/10.1038/s41467-022-30228-4
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 13
Abstract
PAM requirement is a constraint for genome editing but this has been circumvented by engineered Cas9 nucleases as SpG and SpRY recognizing minimal PAM sequences. Here, the authors validate and optimize SpG and SpRY in vivo expanding the targeting landscape in animals.
