Gene editing enables rapid engineering of complex antibiotic assembly lines

Wei Li Thong; Yingxin Zhang; Ying Zhuo; Katherine J. Robins; Joanna K. Fyans; Abigail J. Herbert; Brian J. C. Law; Jason Micklefield

doi:10.1038/s41467-021-27139-1

Nature Communications (Nov 2021)

Gene editing enables rapid engineering of complex antibiotic assembly lines

Wei Li Thong,
Yingxin Zhang,
Ying Zhuo,
Katherine J. Robins,
Joanna K. Fyans,
Abigail J. Herbert,
Brian J. C. Law,
Jason Micklefield

Affiliations

Wei Li Thong: Department of Chemistry and Manchester Institute of Biotechnology, The University of Manchester
Yingxin Zhang: Department of Chemistry and Manchester Institute of Biotechnology, The University of Manchester
Ying Zhuo: Department of Chemistry and Manchester Institute of Biotechnology, The University of Manchester
Katherine J. Robins: Department of Chemistry and Manchester Institute of Biotechnology, The University of Manchester
Joanna K. Fyans: Department of Chemistry and Manchester Institute of Biotechnology, The University of Manchester
Abigail J. Herbert: Department of Chemistry and Manchester Institute of Biotechnology, The University of Manchester
Brian J. C. Law: Department of Chemistry and Manchester Institute of Biotechnology, The University of Manchester
Jason Micklefield: Department of Chemistry and Manchester Institute of Biotechnology, The University of Manchester

DOI: https://doi.org/10.1038/s41467-021-27139-1
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 10

Abstract

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Engineering biosynthetic assembly lines is a powerful path to new natural products but is challenging with current methods. Here the authors use CRISPR-Cas9 to exchange subdomains within NRPS to alter substrate selectivity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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