CRISPR Screening: Molecular Tools for Studying Virus–Host Interactions

Abstract

CRISPR/Cas is a powerful tool for studying the role of genes in viral infections. The invention of CRISPR screening technologies has made it possible to untangle complex interactions between the host and viral agents. Moreover, whole-genome and pathway-specific CRISPR screens have facilitated identification of novel drug candidates for treating viral infections. In this review, we highlight recent developments in the fields of CRISPR/Cas with a focus on the use of CRISPR screens for studying viral infections and identifying new candidate genes to aid development of antivirals.

Keywords

• SARS-CoV-2
• COVID-19
• hepatitis
• HIV
• Cas12
• Cas9