Comparisons and genetic assessments of WSSV resistance and growth in strain cross of Litopenaeus vannamei

Qiang Fu; Kun Sun; Juan Sui; Xupeng Li; Jiawang Cao; Jian Tan; Baolong Chen; Kun Luo; Sheng Luan; Jie Kong; Xianhong Meng

Aquaculture Reports (Jun 2023)

Comparisons and genetic assessments of WSSV resistance and growth in strain cross of Litopenaeus vannamei

Qiang Fu,
Kun Sun,
Juan Sui,
Xupeng Li,
Jiawang Cao,
Jian Tan,
Baolong Chen,
Kun Luo,
Sheng Luan,
Jie Kong,
Xianhong Meng

Affiliations

Qiang Fu: National Key Laboratory of Mariculture Biobreeding and Sustainable Goods (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science), Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao 266071, China
Kun Sun: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Juan Sui: National Key Laboratory of Mariculture Biobreeding and Sustainable Goods (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science), Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao 266071, China
Xupeng Li: National Key Laboratory of Mariculture Biobreeding and Sustainable Goods (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science), Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao 266071, China
Jiawang Cao: National Key Laboratory of Mariculture Biobreeding and Sustainable Goods (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science), Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao 266071, China
Jian Tan: National Key Laboratory of Mariculture Biobreeding and Sustainable Goods (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science), Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao 266071, China
Baolong Chen: National Key Laboratory of Mariculture Biobreeding and Sustainable Goods (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science), Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao 266071, China
Kun Luo: National Key Laboratory of Mariculture Biobreeding and Sustainable Goods (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science), Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao 266071, China
Sheng Luan: National Key Laboratory of Mariculture Biobreeding and Sustainable Goods (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science), Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao 266071, China
Jie Kong: National Key Laboratory of Mariculture Biobreeding and Sustainable Goods (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science), Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao 266071, China
Xianhong Meng: National Key Laboratory of Mariculture Biobreeding and Sustainable Goods (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science), Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao 266071, China; Correspondence to: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China.

Journal volume & issue: Vol. 30
p. 101572

Abstract

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To explore the performance differences among offspring obtained by the intra- and inter-strain crosses, strain A with outstanding WSSV resistance and strain B with outstanding growth of Litopenaeus vannamei were used to construct four experimental groups in a complete diallel cross. 27 growth-related phenotypic traits and WSSV resistance represented by the surviving hours post-infection were obtained via the growth and the virus challenge test. Mixed linear model along with physical pedigree were utilized to calculate the least squares means, correlations, heritability and heterosis. The results showed that crossbreeding can realize character complementation of advantage strain. The performance of WSSV resistance was ranked AA> BA> AB> BB, and the reciprocal crosses performed significantly better than intra-strain group BB. Most growth-related traits in two reciprocal cross groups were also significantly better than those in AA group. Comparisons between the two reciprocal crosses showed that BA cross was better than AB in terms of WSSV resistance and growth. Heterosis of WSSV resistance in BA cross was 5.74∼10.18% greater than that in AB, meanwhile the absolute weight gain rate of BA cross was 4.83% higher than that of AB. The heritability estimates of body length and body weight were 0.0278 and 0.339, both of which reached medium level. The genetic and phenotypic correlations among growth-related traits were from 0.36 to 0.99 and 0.32 to 0.99, respectively. In term of WSSV resistance, the heritability estimate was 0.135 ± 0.082. The genetic and phenotypic correlations between WSSV resistance and growth-related traits were from − 0.034 to − 0.573 and − 0.001 to − 0.202, respectively. The results showed that hybrid offspring obtained by crossing female parents with outstanding WSSV resistance and male parents with outstanding growth culminated in a superior heterosis in both traits. This study provides basic reference data for L. vannamei breeding of new strains that are disease resistant and fast-growing.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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