Nature Communications (Feb 2019)

Barcode-free next-generation sequencing error validation for ultra-rare variant detection

  • Huiran Yeom,
  • Yonghee Lee,
  • Taehoon Ryu,
  • Jinsung Noh,
  • Amos Chungwon Lee,
  • Han-Byoel Lee,
  • Eunji Kang,
  • Seo Woo Song,
  • Sunghoon Kwon

DOI
https://doi.org/10.1038/s41467-019-08941-4
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 8

Abstract

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Next generation sequencing has difficulty in detecting rare-frequency variants due to high sequencing errors. Here the authors present a barcode-free error validation method that physically extracts erroneous reads to identify true variants.