Analysis of Sugar Component of a Hot Water Extract from Arabidopsis thaliana Pollen Tubes Using GC-EI-MS
Marie Dumont,
Arnaud Lehner,
Corinne Loutelier-Bourhis,
Jean-Claude Mollet,
Patrice Lerouge
Affiliations
Marie Dumont
Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV) EA4358, Normandy University, University of Rouen, Institut de Recherche et d’Innovation Biomédicale, Mont-Saint-Aignan, France
Arnaud Lehner
Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV) EA4358, Normandy University, University of Rouen, Institut de Recherche et d’Innovation Biomédicale, Mont-Saint-Aignan, France
Corinne Loutelier-Bourhis
Normandie Université, COBRA, Université de Rouen, INSA de Rouen, CNRS, IRCOF, Mont-Saint-Aignan, France
Jean-Claude Mollet
Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV) EA4358, Normandy University, University of Rouen, Institut de Recherche et d’Innovation Biomédicale, Mont-Saint-Aignan, France
Patrice Lerouge
Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV) EA4358, Normandy University, University of Rouen, Institut de Recherche et d’Innovation Biomédicale, Mont-Saint-Aignan, France
Extraction with hot water is the oldest and simplest method used to recover pectin from an alcohol insoluble residue extract, although this method has not been widely used for the cell wall analysis of pollen tube, a model used to study cell wall. This protocol described this method applied for pectin extraction from 6 h-old Arabidopsis pollen tubes followed by a sugar composition analysis by gas chromatography mass spectrometry.
