Foods (Nov 2024)

Anti-Melanogenic Activities of <i>Sargassum fusiforme</i> Polyphenol-Rich Extract on α-MSH-Stimulated B16F10 Cells via PI3K/Akt and MAPK/ERK Pathways

  • Bei Chen,
  • Honghong Chen,
  • Kun Qiao,
  • Min Xu,
  • Jingna Wu,
  • Yongchang Su,
  • Yan Shi,
  • Lina Ke,
  • Zhiyu Liu,
  • Qin Wang

DOI
https://doi.org/10.3390/foods13223556
Journal volume & issue
Vol. 13, no. 22
p. 3556

Abstract

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Background: Melanin overproduction leads to pigmented skin diseases. Brown algae polyphenols, non-toxic secondary metabolites, exhibit potential bioactivities. Sargassum fusiforme, an edible seaweed, has been underexplored in the field of beauty despite its polyphenol richness. Methods: Polyphenols from S. fusiforme were extracted using macroporous resin (SFRP) and ethyl acetate (SFEP). Their antioxidant and anti-aging properties, tyrosinase inhibitory activities, and mechanisms were assessed. The melanogenesis inhibition effect and mechanism by SFRP was examined in B16F10 melanoma cells. Results: Both SFRP and SFEP demonstrated scavenging activities against DPPH, superoxide anion, and hydroxyl radicals. SFRP showed stronger anti-collagenase and anti-elastase effects. They dose-dependently inhibited mushroom tyrosinase, with IC50 values of 9.89 μg/mL for SFRP and 0.99 μg/mL for SFEP. SFRP reversibly inhibited tyrosinase, while SFEP showed irreversible inhibition. SFRP also suppressed melanin content and intracellular tyrosinase activity in B16F10 cells, downregulating the expression of microphthalmia-associated transcription factor, tyrosinase, and tyrosinase-related protein 1 and 2 expression through the PI3K/Akt and MAPK/ERK signal pathways. Conclusions: S. fusiforme polyphenols, especially SFRP, exhibit promising antioxidant, anti-aging, and melanogenesis inhibitory properties, highlighting their potential application as novel anti-melanogenic agents in cosmetics and the food industry.

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