Generation of heterozygous (MCRIi030-A-1) and homozygous (MCRIi030-A-2) NR2F2/COUP-TFII knockout human iPSC lines

Lucas G.A. Ferreira; Mauricio C. Cabral-da-Silva; Svenja Pachernegg; Jocelyn A. van den Bergen; Gorjana Robevska; Katerina Vlahos; Sara E. Howden; Elizabeth S. Ng; Magnus R. Dias-da-Silva; Andrew H. Sinclair; Katie L. Ayers

Stem Cell Research (Apr 2024)

Generation of heterozygous (MCRIi030-A-1) and homozygous (MCRIi030-A-2) NR2F2/COUP-TFII knockout human iPSC lines

Lucas G.A. Ferreira,
Mauricio C. Cabral-da-Silva,
Svenja Pachernegg,
Jocelyn A. van den Bergen,
Gorjana Robevska,
Katerina Vlahos,
Sara E. Howden,
Elizabeth S. Ng,
Magnus R. Dias-da-Silva,
Andrew H. Sinclair,
Katie L. Ayers

Affiliations

Lucas G.A. Ferreira: Laboratory of Molecular and Translational Endocrinology (LEMT), Endocrinology Division, Department of Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil; The Murdoch Children’s Research Institute, Melbourne, Australia
Mauricio C. Cabral-da-Silva: The Murdoch Children’s Research Institute, Melbourne, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Murdoch Children’s Research Institute, Melbourne, Australia
Svenja Pachernegg: The Murdoch Children’s Research Institute, Melbourne, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Australia
Jocelyn A. van den Bergen: The Murdoch Children’s Research Institute, Melbourne, Australia
Gorjana Robevska: The Murdoch Children’s Research Institute, Melbourne, Australia
Katerina Vlahos: The Murdoch Children’s Research Institute, Melbourne, Australia
Sara E. Howden: The Murdoch Children’s Research Institute, Melbourne, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Murdoch Children’s Research Institute, Melbourne, Australia
Elizabeth S. Ng: The Murdoch Children’s Research Institute, Melbourne, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Murdoch Children’s Research Institute, Melbourne, Australia
Magnus R. Dias-da-Silva: Laboratory of Molecular and Translational Endocrinology (LEMT), Endocrinology Division, Department of Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil
Andrew H. Sinclair: The Murdoch Children’s Research Institute, Melbourne, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Australia
Katie L. Ayers: The Murdoch Children’s Research Institute, Melbourne, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Australia; Corresponding author at: The Murdoch Children’s Research Institute, Melbourne, Australia.

Journal volume & issue: Vol. 76
p. 103374

Abstract

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The NR2F2 gene encodes the transcription factor COUP-TFII, which is upregulated in embryonic mesoderm. Heterozygous variants in NR2F2 cause a spectrum of congenital anomalies including cardiac and gonadal phenotypes. We generated heterozygous (MCRIi030-A-1) and homozygous (MCRIi030-A-2) NR2F2-knockout induced pluripotent stem cell (iPSC) lines from human fibroblasts using a one-step protocol for CRISPR/Cas9 gene-editing and episomal-based reprogramming. Both iPSC lines exhibited a normal karyotype, typical pluripotent cell morphology, pluripotency marker expression, and the capacity to differentiate into the three embryonic germ layers. These lines will allow us to explore the role of NR2F2 during development and disease.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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