Oxidized low-density lipoprotein changes the inflammatory status and metabolomics profiles in human and mouse macrophages and microglia
Yaru Sun,
Jia-Jian Liang,
Jianming Xu,
Kewen Zhou,
Changzhen Fu,
Shao-Lang Chen,
Rucui Yang,
Tsz Kin Ng,
Qingping Liu,
Mingzhi Zhang
Affiliations
Yaru Sun
Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Shantou University Medical College, Shantou, Guangdong, China
Jia-Jian Liang
Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China
Jianming Xu
Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Shantou University Medical College, Shantou, Guangdong, China
Kewen Zhou
Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Shantou University Medical College, Shantou, Guangdong, China
Changzhen Fu
Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China
Shao-Lang Chen
Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China
Rucui Yang
Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Shantou University Medical College, Shantou, Guangdong, China
Tsz Kin Ng
Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong
Qingping Liu
Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Corresponding author.
Mingzhi Zhang
Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Corresponding author.
The conjunctiva of primary open angle glaucoma patients showed high level of oxidized low-density lipoprotein (ox-LDL), which is associated with the inflammatory response. Microglia and macrophages are the immune cells involved in retinal ganglion cell survival regulation; yet, their roles of the ox-LDL-induced inflammation in glaucoma remain elusive. Here we aimed to investigate the lipid uptake, inflammatory cytokine expression, and metabolomics profiles of human and murine-derived microglial and macrophage cell lines treated with ox-LDL. Under the same ox-LDL concentration, macrophages exhibited higher lipid uptake and expression of pro-inflammatory cytokines as compared to microglia. The ox-LDL increased the levels of fatty acid metabolites in macrophages and sphingomyelin metabolites in microglia. In summary, this study revealed the heterogeneity in the inflammatory capacity and metabolic profiles of macrophages and microglia under the stimulation of ox-LDL.
