Oxidized low-density lipoprotein changes the inflammatory status and metabolomics profiles in human and mouse macrophages and microglia

Yaru Sun; Jia-Jian Liang; Jianming Xu; Kewen Zhou; Changzhen Fu; Shao-Lang Chen; Rucui Yang; Tsz Kin Ng; Qingping Liu; Mingzhi Zhang

Heliyon (Apr 2024)

Oxidized low-density lipoprotein changes the inflammatory status and metabolomics profiles in human and mouse macrophages and microglia

Yaru Sun,
Jia-Jian Liang,
Jianming Xu,
Kewen Zhou,
Changzhen Fu,
Shao-Lang Chen,
Rucui Yang,
Tsz Kin Ng,
Qingping Liu,
Mingzhi Zhang

Affiliations

Yaru Sun: Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Shantou University Medical College, Shantou, Guangdong, China
Jia-Jian Liang: Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China
Jianming Xu: Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Shantou University Medical College, Shantou, Guangdong, China
Kewen Zhou: Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Shantou University Medical College, Shantou, Guangdong, China
Changzhen Fu: Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China
Shao-Lang Chen: Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China
Rucui Yang: Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Shantou University Medical College, Shantou, Guangdong, China
Tsz Kin Ng: Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong
Qingping Liu: Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Corresponding author.
Mingzhi Zhang: Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Gaungdong, China; Corresponding author.

Journal volume & issue: Vol. 10, no. 7
p. e28806

Abstract

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The conjunctiva of primary open angle glaucoma patients showed high level of oxidized low-density lipoprotein (ox-LDL), which is associated with the inflammatory response. Microglia and macrophages are the immune cells involved in retinal ganglion cell survival regulation; yet, their roles of the ox-LDL-induced inflammation in glaucoma remain elusive. Here we aimed to investigate the lipid uptake, inflammatory cytokine expression, and metabolomics profiles of human and murine-derived microglial and macrophage cell lines treated with ox-LDL. Under the same ox-LDL concentration, macrophages exhibited higher lipid uptake and expression of pro-inflammatory cytokines as compared to microglia. The ox-LDL increased the levels of fatty acid metabolites in macrophages and sphingomyelin metabolites in microglia. In summary, this study revealed the heterogeneity in the inflammatory capacity and metabolic profiles of macrophages and microglia under the stimulation of ox-LDL.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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