OncoTargets and Therapy (May 2019)

LncRNA GAS5-mediated miR-1323 promotes tumor progression by targeting TP53INP1 in hepatocellular carcinoma

  • Zhang F,
  • Yang C,
  • Xing Z,
  • Liu P,
  • Zhang B,
  • Ma X,
  • Huang L,
  • Zhuang L

Journal volume & issue
Vol. Volume 12
pp. 4013 – 4023

Abstract

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Fengjuan Zhang,1 Chao Yang,1 Zhiyuan Xing,2 Pei Liu,1 Bo Zhang,3 Xiang Ma,4 Liuye Huang,3 Likun Zhuang51Department of Infectious Diseases, The Affiliated Hospital of Qingdao University, Qingdao 266003, People’s Republic of China; 2Department of General Surgery, The Second Affiliated Hospital of Qingdao University, Qingdao 266003, People’s Republic of China; 3Department of Gastroenterology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, People’s Republic of China; 4Department of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qingdao University, Qingdao 266003, People’s Republic of China; 5Institute of Transplantation Science, The Affiliated Hospital of Qingdao University, Qingdao 266003, People’s Republic of ChinaBackground: MiR-1323 was identified in 2006. Until now, the roles and mechanisms of miR-1323 in the progression of cancers including hepatocellular carcinoma (HCC) remain unknown. The aim of this study was to investigate the expressions, roles and mechanisms of miR-1323 in HCC development.Methods: QRT-PCR was used to evaluate the expressions of miR-1323, GAS5 and TP53INP1 in HCC tissues and cell lines. CCK-8 assay, transwell invasion assay and flow cytometry assay were conducted to evaluate the proliferation, invasion and apoptosis of HCC cells. Luciferase assay was used to identify microRNA-target interaction.Results: Firstly, our results showed that miR-1323 promoted proliferation and invasion, and inhibited apoptosis of HCC cells. Secondly, we found that TP53INP1 was a direct target of miR-1323 and could reverse the effects of miR-1323 on proliferation, invasion and apoptosis of HCC cells. Thirdly, our results showed that long non-coding RNA (lncRNA) GAS5 and miR-1323 could interact with each other and affect biological processes of HCC cells. Furthermore, we identified the negative correlations between miR-1323 and TP53INP1, and between miR-1323 and GAS5 in tumor tissues of patients with HCC.Conclusion: Taken together, our study revealed the important roles of GAS5/miR-1323/TP53INP1 axis in HCC progression. This study also provided promising strategies for targeted therapy of patients with HCC.Keywords: miR-1323, TP53INP1, GAS5, HCC, cell proliferation and invasion

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