Modelling of the Citric Acid Production from Crude Glycerol by Wild-Type <i>Yarrowia lipolytica</i> DSM 8218 Using Response Surface Methodology (RSM)
Romina Giacomobono,
Roberto Albergo,
Vito Valerio,
Antonio Caporusso,
Isabella De Bari
Affiliations
Romina Giacomobono
Laboratory for Processes and Technologies for Biorefineries and Green Chemistry, Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), C.R. Trisaia S.S. 106 Jonica, 75026 Rotondella, Italy
Roberto Albergo
Laboratory for Processes and Technologies for Biorefineries and Green Chemistry, Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), C.R. Trisaia S.S. 106 Jonica, 75026 Rotondella, Italy
Vito Valerio
Laboratory for Processes and Technologies for Biorefineries and Green Chemistry, Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), C.R. Trisaia S.S. 106 Jonica, 75026 Rotondella, Italy
Antonio Caporusso
Laboratory for Processes and Technologies for Biorefineries and Green Chemistry, Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), C.R. Trisaia S.S. 106 Jonica, 75026 Rotondella, Italy
Isabella De Bari
Laboratory for Processes and Technologies for Biorefineries and Green Chemistry, Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), C.R. Trisaia S.S. 106 Jonica, 75026 Rotondella, Italy
Crude glycerol is the main by-product of the biodiesel manufacturing industry (10% w/w). Its use as a substrate in microbial fermentations is a concrete strategy to efficiently address its market surplus. In this study, the conversion of crude glycerol to citric acid, a key biochemical in the emerging bioeconomy, by a wild-type yeast Yarrowia lipolytica DSM 8218 was modelled using the Response Surface Methodology. The model relates C/N mass ratio and crude glycerol concentration to maximize the citric acid yield in flask scale using two different N sources, yeast extract and ammonium sulphate. Under the optimal conditions (yeast extract, C/N 141, glycerol 33 g/L), the conversion yield was 0.249 g/g. The optimal conditions were used for up-scaling a fed-batch fermentation in a 2 L bioreactor highlighting a metabolic shift from mannitol to citric acid when high stirring rates were applied (800 rpm). In these conditions, a morphic transition from pseudo-mycelial form to round-shaped yeast-like cells was observed too.
