PLoS ONE (Jan 2015)
Comparative genomics of Cluster O mycobacteriophages.
- Steven G Cresawn,
- Welkin H Pope,
- Deborah Jacobs-Sera,
- Charles A Bowman,
- Daniel A Russell,
- Rebekah M Dedrick,
- Tamarah Adair,
- Kirk R Anders,
- Sarah Ball,
- David Bollivar,
- Caroline Breitenberger,
- Sandra H Burnett,
- Kristen Butela,
- Deanna Byrnes,
- Sarah Carzo,
- Kathleen A Cornely,
- Trevor Cross,
- Richard L Daniels,
- David Dunbar,
- Ann M Findley,
- Chris R Gissendanner,
- Urszula P Golebiewska,
- Grant A Hartzog,
- J Robert Hatherill,
- Lee E Hughes,
- Chernoh S Jalloh,
- Carla De Los Santos,
- Kevin Ekanem,
- Sphindile L Khambule,
- Rodney A King,
- Christina King-Smith,
- Karen Klyczek,
- Greg P Krukonis,
- Christian Laing,
- Jonathan S Lapin,
- A Javier Lopez,
- Sipho M Mkhwanazi,
- Sally D Molloy,
- Deborah Moran,
- Vanisha Munsamy,
- Eddie Pacey,
- Ruth Plymale,
- Marianne Poxleitner,
- Nathan Reyna,
- Joel F Schildbach,
- Joseph Stukey,
- Sarah E Taylor,
- Vassie C Ware,
- Amanda L Wellmann,
- Daniel Westholm,
- Donna Wodarski,
- Michelle Zajko,
- Thabiso S Zikalala,
- Roger W Hendrix,
- Graham F Hatfull
Affiliations
- Steven G Cresawn
- Welkin H Pope
- Deborah Jacobs-Sera
- Charles A Bowman
- Daniel A Russell
- Rebekah M Dedrick
- Tamarah Adair
- Kirk R Anders
- Sarah Ball
- David Bollivar
- Caroline Breitenberger
- Sandra H Burnett
- Kristen Butela
- Deanna Byrnes
- Sarah Carzo
- Kathleen A Cornely
- Trevor Cross
- Richard L Daniels
- David Dunbar
- Ann M Findley
- Chris R Gissendanner
- Urszula P Golebiewska
- Grant A Hartzog
- J Robert Hatherill
- Lee E Hughes
- Chernoh S Jalloh
- Carla De Los Santos
- Kevin Ekanem
- Sphindile L Khambule
- Rodney A King
- Christina King-Smith
- Karen Klyczek
- Greg P Krukonis
- Christian Laing
- Jonathan S Lapin
- A Javier Lopez
- Sipho M Mkhwanazi
- Sally D Molloy
- Deborah Moran
- Vanisha Munsamy
- Eddie Pacey
- Ruth Plymale
- Marianne Poxleitner
- Nathan Reyna
- Joel F Schildbach
- Joseph Stukey
- Sarah E Taylor
- Vassie C Ware
- Amanda L Wellmann
- Daniel Westholm
- Donna Wodarski
- Michelle Zajko
- Thabiso S Zikalala
- Roger W Hendrix
- Graham F Hatfull
- DOI
- https://doi.org/10.1371/journal.pone.0118725
- Journal volume & issue
-
Vol. 10,
no. 3
p. e0118725
Abstract
Mycobacteriophages--viruses of mycobacterial hosts--are genetically diverse but morphologically are all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group of five closely related mycobacteriophages--Corndog, Catdawg, Dylan, Firecracker, and YungJamal--designated as Cluster O with long flexible tails but with unusual prolate capsids. Proteomic analysis of phage Corndog particles, Catdawg particles, and Corndog-infected cells confirms expression of half of the predicted gene products and indicates a non-canonical mechanism for translation of the Corndog tape measure protein. Bioinformatic analysis identifies 8-9 strongly predicted SigA promoters and all five Cluster O genomes contain more than 30 copies of a 17 bp repeat sequence with dyad symmetry located throughout the genomes. Comparison of the Cluster O phages provides insights into phage genome evolution including the processes of gene flux by horizontal genetic exchange.