Adaptive Evolution of New Variants of Dengue Virus Serotype 1 Genotype V Circulating in the Brazilian Amazon
Geovani de Oliveira Ribeiro,
Danielle Elise Gill,
Edcelha Soares D’Athaide Ribeiro,
Fred Julio Costa Monteiro,
Vanessa S. Morais,
Roberta Marcatti,
Marlisson Octavio da S. Rego,
Emerson Luiz Lima Araújo,
Steven S. Witkin,
Fabiola Villanova,
Xutao Deng,
Ester Cerdeira Sabino,
Eric Delwart,
Élcio Leal,
Antonio Charlys da Costa
Affiliations
Geovani de Oliveira Ribeiro
Laboratório de Diversidade Viral, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belem 66075-000, Brazil
Danielle Elise Gill
Departamento de Moléstias Infecciosas e Parasitárias and Instituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, São Paulo 05403-000, Brazil
Edcelha Soares D’Athaide Ribeiro
Public Health Laboratory of Amapa—LACEN/AP, Health Surveillance Superintendence of Amapa, Macapa 68905-230, Brazil
Fred Julio Costa Monteiro
Public Health Laboratory of Amapa—LACEN/AP, Health Surveillance Superintendence of Amapa, Macapa 68905-230, Brazil
Vanessa S. Morais
Departamento de Moléstias Infecciosas e Parasitárias and Instituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, São Paulo 05403-000, Brazil
Roberta Marcatti
Departamento de Moléstias Infecciosas e Parasitárias and Instituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, São Paulo 05403-000, Brazil
Marlisson Octavio da S. Rego
Public Health Laboratory of Amapa—LACEN/AP, Health Surveillance Superintendence of Amapa, Macapa 68905-230, Brazil
Emerson Luiz Lima Araújo
General Coordination of Public Health Laboratories of the Strategic Articulation, Department of the Health Surveillance, Secretariat of the Ministry of Health (CGLAB/DAEVS/SVS-MS), Brasília 70719-040, Brazil
Steven S. Witkin
Department of Obstetrics and Gynecology, Weill Cornell Medicine, New York, NY 10065-4850, USA
Fabiola Villanova
Laboratório de Diversidade Viral, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belem 66075-000, Brazil
Xutao Deng
Vitalant Research Institute, 270 Masonic Avenue, San Francisco, CA 94118-4417, USA
Ester Cerdeira Sabino
Departamento de Moléstias Infecciosas e Parasitárias and Instituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, São Paulo 05403-000, Brazil
Eric Delwart
Vitalant Research Institute, 270 Masonic Avenue, San Francisco, CA 94118-4417, USA
Élcio Leal
Laboratório de Diversidade Viral, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belem 66075-000, Brazil
Antonio Charlys da Costa
Departamento de Moléstias Infecciosas e Parasitárias and Instituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, São Paulo 05403-000, Brazil
Dengue virus (DENV) is a mosquito-borne viral pathogen that plagues many tropical-climate nations around the world, including Brazil. Molecular epidemiology is a growing and increasingly invaluable tool for understanding the dispersal, persistence, and diversity of this impactful virus. In this study, plasma samples (n = 824) from individuals with symptoms consistent with an arboviral febrile illness were analyzed to identity the molecular epidemiological dynamics of DENV circulating in the Brazilian state of Amapá. Twelve DENV type 1 (DENV-1) genomes were identified, which were phylogenetically related to the BR4 lineage of genotype V. Phylodynamics analysis suggested that DENV-1 BR-4 was introduced into Amapá around early 2010, possibly from other states in northern Brazil. We also found unique amino acids substitutions in the DENV-1 envelope and NS5 protein sequences in the Amapá isolates. Characterization of the DENV-1 BR-4 sequences highlights the potential of this new lineage to drive outbreaks of dengue in the Amazon region.
