In vivo real-time monitoring of anti-factor Xa level using a microdialysis-coupled microfluidic device
Xingbei Zhou,
Tao Ding,
Shushui Wang,
Longping Wen
Affiliations
Xingbei Zhou
School of Medicine, South China University of Technology, Guangzhou, Guangdong, 510006, China; Department of Pediatric Cardiology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangdong Cardiovascular Institute, Guangzhou, Guangdong, 510080, China
Tao Ding
School of Medicine, South China University of Technology, Guangzhou, Guangdong, 510006, China
Shushui Wang
Department of Pediatric Cardiology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangdong Cardiovascular Institute, Guangzhou, Guangdong, 510080, China; Co-Corresponding author at: Department of Pediatric Cardiology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangdong Cardiovascular Institute, Guangzhou, Guangdong, 510080, China.
Longping Wen
School of Medicine, South China University of Technology, Guangzhou, Guangdong, 510006, China; Nanobio Laboratory, Institute of Life Sciences, South China University of Technology, Guangzhou, Guangdong, 510006, China; Corresponding author at: School of Medicine, South China University of Technology, Guangzhou, Guangdong 510006, China.
The feasibility of in vivo real-time monitoring of anti-factor Xa level had been explored using an integrated microdialysis-coupled microfluidic system. By customizing the dialysis membrane and utilizing a fluorogenic substrate, the performance of the integrated device was characterized and tested by continuous monitoring of the anti-factor Xa level in rats. Efficient sampling for low molecular weight heparin in the blood stream was demonstrated and the detection range of the device was determined to be 0.1-1.5 IU/ml. The anti-factor Xa profile was recorded for a period of 7 hours. Moreover, the results generated from this method agreed well with those from conventional assay. The present system provides a novel quantitative monitoring method with great precision, good reproducibility and full data coverage of treatment period. This study has paved the road for developing a bedside drug monitoring device in hospital setting.
