Rapid and Sensitive Detection of Polycyclic Aromatic Hydrocarbons in Tea Leaves Using Magnetic Approach
Xiaohan Zhao,
Xiao Feng,
Jingwen Chen,
Lanxin Zhang,
Lingzi Zhai,
Sizhe Lv,
Yonghao Ye,
Yongqi Chen,
Tian Zhong,
Xi Yu,
Ying Xiao
Affiliations
Xiaohan Zhao
State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao 999078, China
Xiao Feng
Key Laboratory of Grains and Oils Quality Control and Processing, Collaborative Innovation Center for Modern Grain Circulation and Safety, College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China
Jingwen Chen
Faculty of Medicine, Macau University of Science and Technology, Avenida Wai Long Taipa, Macao 999078, China
Lanxin Zhang
Faculty of Medicine, Macau University of Science and Technology, Avenida Wai Long Taipa, Macao 999078, China
Lingzi Zhai
Faculty of Medicine, Macau University of Science and Technology, Avenida Wai Long Taipa, Macao 999078, China
Sizhe Lv
Faculty of Medicine, Macau University of Science and Technology, Avenida Wai Long Taipa, Macao 999078, China
Yonghao Ye
Zhuhai Resproly Pharmaceutical Technology Co., Ltd., Blk 11, International Health Port, No. 628, Airport West Road, Jinwan District, Zhuhai 519040, China
Yongqi Chen
Zhuhai Resproly Pharmaceutical Technology Co., Ltd., Blk 11, International Health Port, No. 628, Airport West Road, Jinwan District, Zhuhai 519040, China
Tian Zhong
Faculty of Medicine, Macau University of Science and Technology, Avenida Wai Long Taipa, Macao 999078, China
Xi Yu
Faculty of Medicine, Macau University of Science and Technology, Avenida Wai Long Taipa, Macao 999078, China
Ying Xiao
State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao 999078, China
A rapid and efficient method using an alkyl-functionalized magnetic nanoparticles-based extraction technique combined with Ultra-High Performance Liquid Chromatography was developed for the detection of trace amounts of polycyclic aromatic hydrocarbons in tea leaves. As a popular coating for chromatographic column packing materials, C18-alkyl has been demonstrated to be effective in separating polycyclic aromatic hydrocarbons. Additionally, the magnetism of the nanomaterials accelerates the extraction process while their high surface ratio enables desirable dispersity in the sample matrix. Meanwhile, the adsorbents can be washed and reused 30 times without compromising recovery, which greatly reduces the budget. The effects of various parameters were investigated and optimized, and the recoveries for five analytes were in the range of 84.8–105.4%. The RSD of intra-day and inter-day were below 11.9% and 6.8%, respectively. The limits of detection and limits of quantification ranged from 1.69–9.97 ng g−1 and 5.12–30.21 ng g−1, indicating satisfactory sensitivity. Thus, the proposed methodology is rapid, highly efficient, and economical, and it expands the application of magnetic cleanup approaches in complex food matrices.
