Mechanistic insights into nitrogen-induced changes in pasting characteristics of rice during storage based on proteomics analysis

Hanling Liang; Baiyu Gu; Wentao Sun; Bo Li; Hang Qu; Dongbing Tao; Qi Zhang; Tianyu Wang; Yichao Ma; Yajie Wang; Zhaoxia Wu; Qinghai Zhang

Food Chemistry: X (Dec 2023)

Mechanistic insights into nitrogen-induced changes in pasting characteristics of rice during storage based on proteomics analysis

Hanling Liang,
Baiyu Gu,
Wentao Sun,
Bo Li,
Hang Qu,
Dongbing Tao,
Qi Zhang,
Tianyu Wang,
Yichao Ma,
Yajie Wang,
Zhaoxia Wu,
Qinghai Zhang

Affiliations

Hanling Liang: School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, People’s Republic of China
Baiyu Gu: School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, People’s Republic of China
Wentao Sun: Institute of Plant Nutrition and Environmental Resources, Liaoning Academy of Agricultural Sciences, Shenyang 110161, People’s Republic of China
Bo Li: Institute of Plant Nutrition and Environmental Resources, Liaoning Academy of Agricultural Sciences, Shenyang 110161, People’s Republic of China
Hang Qu: Institute of Plant Nutrition and Environmental Resources, Liaoning Academy of Agricultural Sciences, Shenyang 110161, People’s Republic of China
Dongbing Tao: College of Food Science, Shenyang Agricultural University, Shenyang 110866, People’s Republic of China
Qi Zhang: College of Food Science, Shenyang Agricultural University, Shenyang 110866, People’s Republic of China
Tianyu Wang: College of Food Science, Shenyang Agricultural University, Shenyang 110866, People’s Republic of China
Yichao Ma: College of Food Science, Shenyang Agricultural University, Shenyang 110866, People’s Republic of China
Yajie Wang: School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, People’s Republic of China
Zhaoxia Wu: College of Food Science, Shenyang Agricultural University, Shenyang 110866, People’s Republic of China; Corresponding authors.
Qinghai Zhang: School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, People’s Republic of China; Corresponding authors.

Journal volume & issue: Vol. 20
p. 101018

Abstract

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Nitrogen application delays rice quality deterioration due to changes in its pasting characteristics; however, the underlying mechanisms remain unclear. Using a label-free quantitative proteomics approach, we identified differentially expressed proteins (DEPs) during storage in paddy rice treated with different nitrogen levels. On combining the changes in physiological indicators, high-nitrogen treatment was found to downregulate β-1,3-glucanase, reduce the decomposition of cell wall components, downregulate three proteins involved in starch metabolism, decrease the range of the amylose content and increase the range of the amylopectin, upregulate three proteins related to the lysosomal pathway, and enhance glutelin degradation. In addition, it upregulated three proteins related to flavonoid synthesis, which enhanced the stress response ability of rice, thereby contributing to the stability of biological macromolecules. The discovery of these key DEPs provides potential targets for further control over the deterioration of crop seed storage quality.

Published in Food Chemistry: X

ISSN: 2590-1575 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply; Technology: Chemical technology: Food processing and manufacture
Website: https://www.journals.elsevier.com/food-chemistry-x

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