Legume plants enter a symbiosis with soil nitrogen-fixing bacteria (rhizobia), thereby gaining access to assimilable atmospheric nitrogen. Since this symbiosis is important for agriculture, biofertilizers with effective strains of rhizobia are created for crop legumes to increase their yield and minimize the amounts of mineral fertilizers required. In this work, we sequenced and characterized the genome of Rhizobium ruizarguesonis bv. viciae strain RCAM1022, a component of the ‘Rhizotorfin’ biofertilizer produced in Russia and used for pea (Pisum sativum L.).