Effect of Antibacterial Root Canal Sealer on Persistent Apical Periodontitis
Zheng Wang,
Ge Yang,
Biao Ren,
Yuan Gao,
Xian Peng,
Mingyun Li,
Hockin H.K.Xu,
Qi Han,
Jiyao Li,
Xuedong Zhou,
Lei Cheng
Affiliations
Zheng Wang
State Key Laboratory of Oral Diseases, National Clinical Research Centre for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China
Ge Yang
State Key Laboratory of Oral Diseases, National Clinical Research Centre for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China
Biao Ren
State Key Laboratory of Oral Diseases, National Clinical Research Centre for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China
Yuan Gao
State Key Laboratory of Oral Diseases, National Clinical Research Centre for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China
Xian Peng
State Key Laboratory of Oral Diseases, National Clinical Research Centre for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China
Mingyun Li
State Key Laboratory of Oral Diseases, National Clinical Research Centre for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China
Hockin H.K.Xu
Department of Advanced Oral Sciences and Therapeutics, Biomaterials & Tissue Engineering Division, School of Dentistry, University of Maryland, Baltimore, MD 21201, USA
Qi Han
State Key Laboratory of Oral Diseases, National Clinical Research Centre for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China
Jiyao Li
State Key Laboratory of Oral Diseases, National Clinical Research Centre for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China
Xuedong Zhou
State Key Laboratory of Oral Diseases, National Clinical Research Centre for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China
Lei Cheng
State Key Laboratory of Oral Diseases, National Clinical Research Centre for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China
The infection of Enterococcus faecalis and its interacting microorganisms in the root canal could cause persistent apical periodontitis (AP). Antibacterial root canal sealer has favorable prospects to inhibit biofilms. The purpose of this study was to investigated the antibacterial effect of root canal sealer containing dimethylaminododecyl methacrylate (DMADDM) on persistent AP in beagle dogs for the first time. Persistent AP was established by a two-step infection with Enterococcus faecalis and multi-bacteria (Enterococcus faecalis, Lactobacillus acidophilus, Actinomycesnaeslundii, Streptococcus gordonii). Root canal sealer containing DMADDM (0%, 1.25%, 2.5%) was used to complete root canal filling. The volume of lesions and inflammatory grade in the apical area were evaluated by cone beam computer tomography (CBCT) and hematoxylin-eosin staining. Both Enterococcus-faecalis- and multi-bacteria-induced persistent AP caused severe apical destruction, and there were no significant differences in pathogenicity between them. DMADDM-modified sealer significantly reduced the volume of periapical lesion and inflammatory grade compared with the control group, among them, the therapeutic effect of the 2.5% group was better than the 1.25% group. In addition, E.faecalis-induced reinfection was more sensitive to the 2.5% group than multi-bacteria reinfection. This study shows that root canal sealer containing DMADDM had a remarkable therapeutic effect on persistent AP, especially on E. faecalis-induced reinfection.
