Surface-enhanced Raman scattering and Plasmon effect for enzymatic bionanocomplexes characterization

Abstract

Cholesterol oxidase (ChOX) enzyme is one of the most important analytical enzyme, used for cholesterol assay in clinical diagnostics as well as food production, and the developing of innovative solutions for improving the selectivity and accuracy of the analysis including bio-nanotechnological approaches is still ongoing. The Surface Plazmon Resonance (SPR) and the surface enhanced Raman scattering (SERS) as specific for nanocurriers effects were observed what enable us to research the oscillation spectra of the ChOX enzyme. The vibrational lines are attributed to chemical functional groups existing in enzyme, for example, amino acids, amide groups as well as for cofactor. For the improving the SERS effect the gold nanoparticles – ChOX bionanocomplexes were analyzed in combination with gold-coating gratings as a promising plazmonic material.