Potential Protective Effect of Hesperidin (Vitamin P) against Glyphosate-Induced Spermatogenesis Damage in Male Rats: Biochemical and Histopathological Findings on Reproductive Parameters
Şükrü Güngör,
Murat Kırıkkulak,
Barış Denk,
Mehmet Fuat Gülhan,
Özay Güleş,
Duygu Budak,
Muhammed Enes İnanç,
Fatih Avdatek,
Deniz Yeni,
Umut Taşdemir
Affiliations
Şükrü Güngör
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur 15030, Türkiye
Murat Kırıkkulak
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, AfyonKocatepe University, Afyonkarahisar 03200, Türkiye
Barış Denk
Department of Biochemistry, Faculty of Veterinary Medicine, AfyonKocatepe University, Afyonkarahisar 03200, Türkiye
Mehmet Fuat Gülhan
Department of Medicinal and Aromatic Plants, Technical Sciences Vocational School, Aksaray University, Aksaray 68100, Türkiye
Özay Güleş
Department of Histology and Embryology, Faculty of Veterinary Medicine, Aydın Adnan Menderes University, Aydın 09016, Türkiye
Duygu Budak
Department of Zootechnics and Animal Nutrition, Faculty of Veterinary Medicine, Aksaray University, Aksaray 68100, Türkiye
Muhammed Enes İnanç
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur 15030, Türkiye
Fatih Avdatek
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, AfyonKocatepe University, Afyonkarahisar 03200, Türkiye
Deniz Yeni
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, AfyonKocatepe University, Afyonkarahisar 03200, Türkiye
Umut Taşdemir
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Ankara University, Ankara 06110, Türkiye
The aim of this study was to investigate the effectiveness of hesperidin (HES) on testicular histopathological changes, biochemical changes, and semen characteristics in rats exposed to glyphosate (GLP). The control group was given a normal diet devoid of GLP and HES, the HES group was given 100 mg/kg/day HES with the normal diet, the GLP group was given GLP at the LD50/10 dose of normal feed, which was 787.85 mg/kg/day, and the GLP + HES group was given normal feed containing 787.85 mg/kg/day LD50/10 dose of GLP in addition to 100 mg/kg/day HES. GLP administration reduced sperm motility, sperm plasma membrane integrity, glutathione levels, and total antioxidant levels in the testicular tissues of rats. Moreover, it caused an increase in right testis and left epididymis weights, abnormal sperm counts, malondialdehyde levels, total oxidant status, and DNA damage. The HES treatment showed curative effects on these parameters. Furthermore, HES was effective in lessening the histopathological damage that was caused by GLP. The results showedthat HES protects spermatological parameters and DNA integrity, improves antioxidant defenses, and lowers the damage and lipid peroxidation caused by GLP in testicular tissue.
