Comparison of the cytotoxic effects of bulk and nanosized CeO2 on lymphocyte cells

Abstract

Cerium(IV) oxide (CeO2) is widely used in industrial fields such as biomedical, glass, electronics, automotive, and pharmacology. In this study, the cytotoxic effects on human peripheral blood cultures of two forms of cerium(IV) oxide with different particle sizes (Bulk-sized Cerium(IV) oxide: BC and Nano-sized Cerium(IV) oxide: NC) in concentrations range of 0.001-200 ppm were investigated. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay were used to determine the cytotoxicity of these forms. According to the test results, it was determined that both forms caused severe cytotoxicity at all concentrations studied. It was observed that cytotoxicity increased with increasing concentration. NCs are more toxic at all concentrations except 100 and 200 ppm concentrations.

Keywords

• cerium (iv) oxide
• biomaterial
• cytotoxicity
• mtt assay
• lymphocyte
• nanoparticle