<i>Agaricus bisporus</i>-Derived Glucosamine Hydrochloride Regulates VEGF through BMP Signaling to Promote Zebrafish Vascular Development and Impairment Repair
Jiarui Sun,
Qici Wu,
Yuxin Wei,
Wei Zhao,
Haokun Lv,
Wei Peng,
Jiayi Zheng,
Yixuan Chen,
Zhengsen Wang,
Yutian Pan,
Yu Xue
Affiliations
Jiarui Sun
The Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou 363000, China
Qici Wu
The Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou 363000, China
Yuxin Wei
The Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou 363000, China
Wei Zhao
The Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou 363000, China
Haokun Lv
The Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou 363000, China
Wei Peng
The Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou 363000, China
Jiayi Zheng
The Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou 363000, China
Yixuan Chen
The Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou 363000, China
Zhengsen Wang
The Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou 363000, China
Yutian Pan
The Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou 363000, China
Yu Xue
The Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou 363000, China
Glucosamine hydrochloride (GAH) is a natural component of glycoproteins present in almost all human tissues and participates in the construction of human tissues and cell membranes. GAH has a wide range of biological activities, particularly in anti-inflammatory and osteogenic damage repair. At present, little is known about how GAH functions in angiogenesis. To determine the role of GAH on vascular development and impairment repair, we used the inhibitors VRI, DMH1, and dorsomorphin (DM) to construct vascular-impaired models in Tg(kdrl: mCherry) transgenic zebrafish. We then treated with GAH and measured its repair effects on vascular impairment through fluorescence intensity, mRNA, and protein expression levels of vascular-specific markers. Our results indicate that GAH promotes vascular development and repairs impairment by regulating the vascular endothelial growth factor (VEGF) signaling pathway through modulation of bone morphogenetic protein (BMP) signaling. This study provides an experimental basis for the development of GAH as a drug to repair vascular diseases.
