Nature Communications (Feb 2023)
The cytoplasmic synthesis and coupled membrane translocation of eukaryotic polyphosphate by signal-activated VTC complex
- Zeyuan Guan,
- Juan Chen,
- Ruiwen Liu,
- Yanke Chen,
- Qiong Xing,
- Zhangmeng Du,
- Meng Cheng,
- Jianjian Hu,
- Wenhui Zhang,
- Wencong Mei,
- Beijing Wan,
- Qiang Wang,
- Jie Zhang,
- Peng Cheng,
- Huanyu Cai,
- Jianbo Cao,
- Delin Zhang,
- Junjie Yan,
- Ping Yin,
- Michael Hothorn,
- Zhu Liu
Affiliations
- Zeyuan Guan
- National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Juan Chen
- National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Ruiwen Liu
- National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Yanke Chen
- Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences
- Qiong Xing
- State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University
- Zhangmeng Du
- National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Meng Cheng
- National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Jianjian Hu
- National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Wenhui Zhang
- National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Wencong Mei
- National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Beijing Wan
- National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Qiang Wang
- National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Jie Zhang
- National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Peng Cheng
- National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Huanyu Cai
- College of Science, Huazhong Agricultural University
- Jianbo Cao
- Public Laboratory of Electron Microscopy, Huazhong Agricultural University
- Delin Zhang
- National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Junjie Yan
- National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Ping Yin
- National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Michael Hothorn
- Structural Plant Biology Laboratory, Department of Plant Scienes, University of Geneva
- Zhu Liu
- National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
- DOI
- https://doi.org/10.1038/s41467-023-36466-4
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 12
Abstract
The VTC complex generate eukaryotic polyphosphate (polyP). Authors here reveal how the signal-activated VTC synthesize cytosolic polyP and transport polyP across vacuolar membrane into the lumen, by using cryo-EM, in-cell NMR, and smFRET analyses.
