Multiple evaluation of the effect of 60Co-γ irradiation sterilization on the quality of Fructus forsythiae

Abstract

The aim of this study was to investigate the effect of 60Co-γ irradiation sterilization on the quality of Fructus forsythiae. The medicinal powder of Fructus forsythiae was used at different doses. Ingredient concentration,high performance liquid chromatography (HPLC) finger print and antibacterial activity of water extract against Staphylococcus aureus were used before and after irradiation for comparison. A near-infrared spectrum (NIR) consistency model of Fructus forsythiae was established by near-infrared spectroscopic methodology and the optimum dose of 60Co-γ irradiation was investigated. The results showed that there was very significant difference (p0.05). The NIR consistency evaluation model was established to determine the irradiated samples. The microbial populations could be reduced to a lower degree at 5 kGy absorbed dose irradiation. 60Co-γ irradiation sterilization would affect the quality of Fructus forsythiae at 10 kGy dose. It is recommended that the irradiation sterilization dose not exceed 5 kGy.

Keywords

• fructus forsythiae
• 60co-γ ray
• radiation sterilization
• finger print
• antibacterial activity
• near infrared spectrum