Datasets for calcium dynamics comparison between the whole-cell and a β-escin based perforated patch configuration in brain slices from adult mice

Abstract

Multiple processes shape calcium signals in neurons. The spatial and temporal dynamics of these signals are determined by various cellular parameters, including the calcium influx, calcium buffering, and calcium extrusion. The different Ca2+ handling properties can be estimated using the ‘added buffer approach’ [1], which is based on a single compartment model of Ca2+ buffering. To use this approach, the cell has to be loaded with a Ca2+ sensitive dye (e.g., fura-2) via the patch pipette, which is usually done in the whole-cell patch clamp configuration. However, determining Ca2+ handling properties can be complex and frequently unsuccessful due to the wash-out of intracellular components (e.g., mobile Ca2+ buffers) during whole-cell patch clamp recordings. We present two Ca2+ imaging datasets from adult substantia nigra dopamine neurons where the 'added buffer approach' was either combined with the 'conventional' whole-cell configuration or with a β-escin based perforated patch clamp configuration. These data can be used to compare the two methods or to draw comparisons with the Ca2+ handling properties of other neuron types. Further details and an in-depth analysis of the new combination of the ‘added buffer approach’ with the β-escin based perforated patch clamp configuration can be found in our companion manuscripts “Analysis of neuronal Ca2+ handling properties by combining perforated patch clamp recordings and the added buffer approach” [2] and “A Simple Method for Getting Standard Error on the Ratiometric Calcium Estimator” [3].

Keywords

• Calcium imaging
• Added buffer approach
• Perforated patch clamp
• Dopamine neuron
• Substantia nigra
• Beta-escin