CHARACTERIZATION OF EGGPLANT ENDOPHYTE BACTERIA AND RHIZOBACTERIA AS WELL AS THEIR ANTAGONISTIC ABILITY AGAINST Ralstonia solanacearum

Abstract

Characterization of eggplant endophyte bacteria and rhizobacteria as well as their antagonistic ability against Ralstonia solanacearum. Bacterial wilt caused by Ralsonia solanacearum is one of important diseases causing severe loses in eggplant production. Various strategies were used to manage bacterial wilt, including planting resistant varieties, soil amandement, and soil solarization. However, management of R. solanacearum in eggplant by using endophytic bacteria and rhizobacteria were not been done that much. The objective of this study was to: (1) characterization of endophytic and rhizobacteria; (2) determines the inhibition ability of endophytic and rhizobacteria isolates against R. solanacearum pathogen on eggplant. The laboratory experiment was arranged in completely randomized design with 5 treatments and 5 replications. The double layer method using yeast peptone glucose agar (YPGA) medium was used in vitro test. Based on the morphological characteristics these isolates were suspected as a member of genus Bacillus. Among the isolates used in this study, TK isolate showed the best capability to inhibit growth of R. solanacearum.

Keywords

• endophytic bacteria
• ralsonia solanacearum
• rhizobacteria